![Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-Immunohistochemistry-NBP1-51945-img0008.jpg "Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_001280099.1. |
| Epitope | C-PRTAQSEETRVWHR |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CAMK2A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 60kDa band observed in Human Cerebellum and approx 50+60kDa in Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively (calculated MW of 55.3kDa according to Human NP_057065.2 and 54.1kDa according to Mouse NP_803126.1 and Rat NP_037052.1 subunit Alpha and 60.4kDa according to Human NP_001280099.1, Mouse : NP_031621.3 and Rat NP_068507.2 subunit beta).. These observed molecular weights are routinely observed by other sources.and were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. IHC-P: Human brain cortex shows granular staining in the periphery of pyramydal cell bodies. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Hayk Simonyan |
IHC | Mouse | 04/30/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CaM Kinase II alpha Antibody (NBP1-51945)Find related products by research area.
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| Gene Symbol | CAMK2A |
| Entrez | |
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![Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134294.jpg "Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342929.jpg "Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342911.jpg "Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342919.jpg "Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - (0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134296.jpg "Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - (0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence")
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342912.jpg "Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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