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Calreticulin Antibody (681233) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was ...read more
Calreticulin was detected in formalin fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981) at 15 µg/mL overnight at 4 °C. Before incubation ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin Monoclonal Antibody (Catalog # MAB38981, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, Flow, KO
Clone
681233
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC38981U-100UG
Alexa Fluor 405
IC38981V-100UG
Alexa Fluor 488
IC38981G-100UG
Alexa Fluor 532
FAB38981X
Alexa Fluor 594
IC38981T-100UG
Alexa Fluor 647
IC38981R-100UG
Alexa Fluor 700
IC38981N-100UG
Alexa Fluor 750
IC38981S-100UG
Allophycocyanin
FAB38981A
Biotin
FAB38981B
DyLight 350
FAB38981D
DyLight 405
FAB38981E
DyLight 488
FAB38981K
DyLight 550
FAB38981L
DyLight 594
FAB38981M
DyLight 650
FAB38981W
DyLight 680
FAB38981Y
DyLight 755
FAB38981Z
FITC
FAB38981F
HRP
FAB38981H
Janelia Fluor 549
FAB38981I
Janelia Fluor 646
FAB38981J
PE
FAB38981P
PerCP
FAB38981C
mFluor Violet 450 SE
FAB38981MFV450
mFluor Violet 500 SE
FAB38981MFV500
mFluor Violet 610 SE
FAB38981MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB38981-SP
Carrier Free
IC38981V-100UG
Carrier Free
IC38981U-100UG
Carrier Free
IC38981T-100UG
Carrier Free
IC38981S-100UG
Carrier Free
IC38981R-100UG
Carrier Free
IC38981N-100UG
Carrier Free
IC38981G-100UG
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Calreticulin Antibody (681233) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Calreticulin
Glu18-Leu417
Accession # P27797
Specificity
Detects human Calreticulin in ELISA. Detects human, mouse and rat Calreticulin in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CALR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB38981 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calreticulin Antibody (681233) [Unconjugated]

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • Endoplasmic reticulum resident protein 60
  • epididymis secretory sperm binding protein Li 99n
  • ERp60
  • FLJ26680
  • grp60
  • HACBP
  • HEL-S-99n
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • Vasostatin

Background

Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calreticulin Antibody (MAB38981)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow Cytometry, ICC.


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(1)
ICC
(1)
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Bioinformatics

Gene Symbol CALR
Uniprot