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C-myc promoter-binding protein Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: C-myc promoter-binding protein Antibody [NBP2-49444] - Staining of human cell line HaCaT shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: C-myc promoter-binding protein Antibody [NBP2-49444] - Staining of human bone marrow shows moderate nuclear positivity in hematopoietic cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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C-myc promoter-binding protein Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DENND4A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
C-myc promoter-binding protein Recombinant Protein Antigen (NBP2-49444PEP)

Reactivity Notes

Mouse (84%), Rat (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for C-myc promoter-binding protein Antibody

  • c-myc promoter binding protein
  • C-myc promoter-binding protein
  • DENN domain-containing protein 4A
  • DENN/MADD domain containing 4A
  • IRLBFLJ33949
  • KIAA0476
  • MYCPBP

Background

DENND4A, also known as C-myc promoter-binding protein, consists of a 209.2 kDa and a 213.7 kDa isoform and is utilized in promoting the conversion of Rab proteins from their inactive GDP-bound form into their active GTP-bound form. This protein is currently being studied for involvement in prostatitis. In transcription pathways, the protein interacts with YWHAQ, YWHAZ, CEBPE, YWHAB, and YWHAG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DENND4A