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Brg1 Antibody (GT2712)

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Genetic Strategies: Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Wild-type (WT) and Brg1 knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 ...read more
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde ...read more
Immunohistochemistry-Frozen: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Brg1 stained by Brg1 ...read more
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Western Blot: Brg1 Antibody (GT2712) [NBP3-13537] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Brg1 antibody [GT2712] (NBP3-13537) diluted at 1:1000.
Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral ...read more
Immunohistochemistry-Paraffin: Brg1 Antibody (GT2712) [NBP3-13537] - Brg1 antibody [GT2712] detects Brg1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
GT2712
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Brg1 Antibody (GT2712) Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Brg1. The exact sequence is proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMARCA4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (84%), Bovine (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Brg1 Antibody (GT2712)

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Brg1 Antibody (NBP3-13537) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-13537

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Bioinformatics

Gene Symbol SMARCA4