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BRAF35 Antibody (PCRP-HMG20B-1B5)

Images

 
Flow cytometric analysis of PFA-fixed HeLa cells. BRAF35 BRAF35 antibody (PCRP-HMG20B-1B5)) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using BRAF35 Mouse Monoclonal Antibody (PCRP-HMG20B-1B5).
Formalin-fixed, paraffin-embedded human colon stained with BRAF35 antibody (PCRP-HMG20B-1B5). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC, IP
Clone
PCRP-HMG20B-1B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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BRAF35 Antibody (PCRP-HMG20B-1B5) Summary

Description
Positive Controls: Jurkat, A431 or HeLa cells. Ubiquitous expression in adult tissues.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Synthetic peptide (exact sequence is proprietary)
Localization
Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HMG20B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug of protein or 1ml of lysate
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for BRAF35 Antibody (PCRP-HMG20B-1B5)

  • BRAF35Sox-like transcriptional factor
  • high-mobility group 20B
  • HMG domain-containing protein 2
  • HMG domain-containing protein HMGX2
  • HMGX2FLJ26127
  • HMGXB2member 1-related
  • SMARCE1R
  • SMARCE1-related protein

Background

BRAF35 is required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMG20B