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Boll Antibody (3E10) - Azide and BSA Free

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Immunohistochemistry-Paraffin: Boll Antibody (3E10) [H00066037-M03] - Analysis of monoclonal antibody to BOLL on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
ELISA: Boll Antibody (3E10) [H00066037-M03] - Detection limit for recombinant GST tagged BOLL is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clone
3E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

Boll Antibody (3E10) - Azide and BSA Free Summary

Immunogen
BOLL (NP_149019, 185 a.a. ~ 283 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY
Specificity
BOLL - bol, boule-like (Drosophila)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BOLL
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00066037-M03 in the following applications:

Reactivity Notes

Other species not tested. Use in Mouse reported in scientific literature (PMID:31963729).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Boll Antibody (3E10) - Azide and BSA Free

  • bol, boule-like (Drosophila)
  • BOULEbol (Drosophila boule homolog)-like
  • protein boule-like

Background

This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Boll Antibody (H00066037-M03)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, Immunohistochemistry.


Filter By Application
IHC-P
(1)
Immunohistochemistry
(1)
All Applications
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Human
(1)
Mouse
(1)
All Species

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Bioinformatics

Gene Symbol BOLL
Entrez
OMIM
Uniprot