Recombinant Human BNP Protein Summary
Description |
A single non-glycosylated polypeptide chain containing 32 amino acids corresponding to BNP Source: E. coli Uniprot ID: P16860 Amino Acid Sequence: SPKMVQGSGC FGRKMDRISS SSGLGCKVLR RH This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
NPPB |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of BNP as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
3.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Alternate Names for Recombinant Human BNP Protein
Background
Brain Natriuretic Peptide is encoded by the BNP gene located on the Chr.1 in humans. It is firstly discovered in the porcine brain and given this name, but the protein is mainly expressed in the cardiac ventricles in human body after the excessive stretching of cardiomyocytes. The gene expresses a 134 a.a. sequence which contains a 1-26 a.a. signal peptide and 27-134 a.a. Natriuretic peptides B, and the BNP is the 32 a.a. C-terminus of natriuretic peptides B. The BNP can be cleaved in 16 chains and the rHuBNP is 1-32. BNP acts as a cardiac hormone with a variety of functions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Additionnaly, it plays a key role in cardiovascular homeostasis, helps restore the body's salt and water balance and improves heart function.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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