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BMP-10 Antibody (654319)

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BMP‑10 was detected in immersion fixed frozen sections of mouse embryonic heart (E13.5) using Rat Anti-Mouse BMP‑10 Monoclonal Antibody (Catalog # MAB6038) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to developing myocardium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
BMP‑10 was detected in immersion fixed frozen sections of mouse embryonic heart (E13.5) using Rat Anti-Mouse BMP‑10 Monoclonal Antibody (Catalog # MAB6038) at 10 µg/mL overnight at 4 °C. Tissue was stained using ...read more
BMP-10 was detected in immersion fixed frozen sections of mouse embryonic heart (15 d.p.c.) using Rat Anti-Mouse BMP-10 Monoclonal Antibody (Catalog # MAB6038) at 25 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC
Clone
654319
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6038-SP
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BMP-10 Antibody (654319) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn314-Arg421
Accession # Q9R229
Specificity
Detects mouse BMP-10 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BMP-10 and less than 10% cross‑reactivity with rhBMP-8a and recombinant mouse (rm) BMP‑8b is observed. No cross-reactivity with rmBMP-3, -4, -5, -6, -7, -9,
-15, rhBMP-1.1, -2, -3b, or the propeptide of rhBMP-10 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
BMP10
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publication using MAB6038.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-10 Antibody (654319)

  • BMP10
  • BMP-10
  • bone morphogenetic protein 10
  • MGC126783

Background

Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 292 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. The mouse BMP-10 proprotein shares 86% and 95% aa sequence identity with human and rat BMP-10, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMP-10 Antibody (MAB6038)(1)


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Bioinformatics

Gene Symbol BMP10
Uniprot