bcl-x Antibody (SPM337) - IHC-Prediluted

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (Uniprot: Q07817)

Cytoplasmic and cell/nuclear membrane

Apoptosis Marker

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.

IgG2a Kappa

Monoclonal

Mouse

BCL2L1

Protein A or G purified

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Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.