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bcl-x Antibody (SPM165) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM165
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34743AF350
Alexa Fluor 405
NBP2-34743AF405
Alexa Fluor 488
NBP2-34743AF488
Alexa Fluor 532
NBP2-34743AF532
Alexa Fluor 594
NBP2-34743AF594
Alexa Fluor 647
NBP2-34743AF647
Alexa Fluor 700
NBP2-34743AF700
Alexa Fluor 750
NBP2-34743AF750
Allophycocyanin
NBP2-34743APC
Biotin
NBP2-34743B
CoraFluor 1
NBP2-34743CL1
DyLight 350
NBP2-34743UV
DyLight 405
NBP2-34743V
DyLight 488
NBP2-34743G
DyLight 550
NBP2-34743R
DyLight 594
NBP2-34743DL594
DyLight 650
NBP2-34743C
DyLight 680
NBP2-34743FR
DyLight 755
NBP2-34743IR
FITC
NBP2-34743F
HRP
NBP2-34743H
Janelia Fluor 525
NBP2-34743JF525
Janelia Fluor 549
NBP2-34743JF549
Janelia Fluor 585
NBP2-34743JF585
Janelia Fluor 635
NBP2-34743JF635
Janelia Fluor 646
NBP2-34743JF646
PE
NBP2-34743PE
PerCP
NBP2-34743PCP
Unconjugated
NBP2-32810-0.1mg
mFluor Violet 450 SE
NBP2-34743MFV450
mFluor Violet 500 SE
NBP2-34743MFV500
mFluor Violet 610 SE
NBP2-34743MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32810-0.1mg
IHC-Prediluted
NBP2-44415
Azide and BSA Free
NBP2-34743-0.1mg
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bcl-x Antibody (SPM165) [Janelia Fluor® 669] Summary

Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (Uniprot: Q07817)
Localization
Cytoplasmic and cell/nuclear membrane
Marker
Apoptosis Marker
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for bcl-x Antibody (SPM165) [Janelia Fluor® 669]

  • Apoptosis regulator Bcl-X;BCL2L;BCL2L1;Bcl-2-like protein 1;BCLX;Bcl-X;BCL-XL/S;PPP1R52
  • BCL2L1
  • Bclx
  • Bcl-x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL2L1