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bcl-x Antibody (SPM165) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM165
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

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bcl-x Antibody (SPM165) [Janelia Fluor® 585] Summary

Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (Uniprot: Q07817)
Localization
Cytoplasmic and cell/nuclear membrane
Marker
Apoptosis Marker
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for bcl-x Antibody (SPM165) [Janelia Fluor® 585]

  • Apoptosis regulator Bcl-X;BCL2L;BCL2L1;Bcl-2-like protein 1;BCLX;Bcl-X;BCL-XL/S;PPP1R52
  • BCL2L1
  • Bclx
  • Bcl-x

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional bcl-x Products

Array NBP2-34743JF585

Research Areas for bcl-x Antibody (NBP2-34743JF585)

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Bioinformatics

Gene Symbol BCL2L1