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Bcl-6 Antibody (CL14612)

Images

 
Staining of human tonsil shows moderate to strong nuclear positivity in germinal center cells.
Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.
Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Staining in A-549 cell line showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Orthogonal Strategies: Analysis in human lymph node and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.
Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
CL14612
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

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Bcl-6 Antibody (CL14612) Summary

Immunogen
This antibody was developed against a Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence of P41182. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (95%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BCL6
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Bcl-6 Antibody (CL14612)

  • B-cell CLL/lymphoma 6
  • B-cell lymphoma 6 protein transcript
  • Bcl-5
  • BCL5B-cell lymphoma 5 protein
  • Bcl6
  • Bcl-6
  • BCL6A
  • cys-his2 zinc finger transcription factor
  • LAZ3
  • LAZ3ZBTB27B-cell lymphoma 6 protein
  • lymphoma-associated zinc finger gene on chromosome 3
  • Protein LAZ-3
  • ZBTB27
  • Zinc finger and BTB domain-containing protein 27
  • Zinc finger protein 51ZNF51
  • zinc finger transcription factor BCL6S
  • ZNF51

Background

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-6 Antibody (NBP3-26873) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Bcl-6 Antibody (NBP3-26873) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCL6