![Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP1-86429-img0004.jpg "Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: DCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRET |
| Predicted Species | Mouse (92%), Rat (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BAG3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BAG3 Antibody (NBP1-86429)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BAG3 |
![Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86429] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86429-img0005.jpg "Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86429] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0014.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human liver.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0007.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human liver.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human kidney.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0008.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human kidney.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human testis.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0009.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human testis.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0010.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86429 (A) shows similar protein distribution across tissues to independent antibody NBP1-86442 (B).](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0011.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86429 (A) shows similar protein distribution across tissues to independent antibody NBP1-86442 (B).")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0012.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0013.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0015.jpg "Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.")
![Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]](https://images.novusbio.com/images2/HSPB8_MAB4987_Western_Blot_9457.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")