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ATP8A1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: ATP8A1 Antibody [NBP2-30403] - Analysis in human cerebral cortex and liver tissues. Corresponding ATP8A1 RNA-seq data are presented for the same tissues.
Western Blot: ATP8A1 Antibody [NBP2-30403] - Analysis in human cell line Daudi.
Immunocytochemistry/ Immunofluorescence: ATP8A1 Antibody [NBP2-30403] - Staining of human cell line PC-3 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATP8A1 Antibody [NBP2-30403] - Staining of human small intestine shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: ATP8A1 Antibody [NBP2-30403] - Staining of human cerebral cortex shows strong membranous and cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: ATP8A1 Antibody [NBP2-30403] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: ATP8A1 Antibody [NBP2-30403] - Staining of human lymph node shows moderate membranous positivity in germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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ATP8A1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVSQSEVI
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP8A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP8A1 Protein (NBP2-30403PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP8A1 Antibody

  • probable phospholipid-transporting ATPase IA
  • ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
  • ATPASEII
  • ATPIA
  • ATPP2

Background

The P-type adenosinetriphosphatases (P-type ATPases) are a family of proteins which use the free energy of ATP hydrolysis to drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily catalyzes transport of heavy metal ions. Another subfamily transports non-heavy metal ions (NMHI). The protein encoded by this gene is a member of the third subfamily of P-type ATPases and acts to transport amphipaths, such as phosphatidylserine. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATP8A1
Uniprot