| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human ATG3 Met1-His287 Accession # Q9NT62 |
| Specificity | Detects endogenous human, mouse and rat ATG3 in Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | ATG3 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
ATG3 (autophagy-related protein 3; also APG3-like and PC3-96) is a ubiquitous 45 kDa member of the ATG3 family of proteins. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the transfer of ATG7-bound ATG8 (known as LC3, GATE16 and GABA-RAP in mammals) to phosphatidylethanolamine, critical for autophagy. Human ATG3 is 314 amino acids in length and contains an active site at Cys264 that forms a thiol ester bond with the C-terminal Gly of ATG8. There are multiple potential isoform variants. Three show a 22, 28 and 35 aa substitution for aa 289‑314, respectively, while a fourth shows an alternate start site at Met88 that may be accompanied by one of the afore mentioned substitutions. Over aa 1‑287, human ATG3 shares 97.9% aa identity with mouse ATG3.
![Immunohistochemistry ATG7 Antibody (683906) [Unconjugated]](https://images.novusbio.com/images2/ATG7_MAB6608_Immunohistochemistry_10631.jpg)
![Simple Western ATG7 Antibody (683906) [Unconjugated]](https://images.novusbio.com/images2/ATG7_MAB6608_Simple_Western_16409.jpg)
![Western Blot ATG7 Antibody (683906) [Unconjugated]](https://images.novusbio.com/images2/ATG7_MAB6608_Western_Blot_11614.jpg)
| Publication using AF5450 | Applications | Species |
|---|---|---|
| VC Chen, YH Hsieh, LJ Chen, TC Hsu, BS Tzang Escitalopram oxalate induces apoptosis in U-87MG cells and autophagy in GBM8401 cells J. Cell. Mol. Med., 2017-11-03;0(0):. 2017-11-03 [PMID: 29105282] (Western Blot, Human) | Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Western_Blot_19121.jpg)
![Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Immunohistochemistry_19158.jpg)
