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ATG10 Antibody (2Q3Q3)

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Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (NBP3-16649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit mAb (NBP3-16649) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
2Q3Q3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATG10 Antibody (2Q3Q3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 121-220 of human ATG10 (Q9H0Y0). RPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ATG10
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATG10 Antibody (2Q3Q3)

  • APG10 autophagy 10-like (S. cerevisiae)
  • APG10
  • APG10L
  • APG10-like
  • ATG10 autophagy related 10 homolog (S. cerevisiae)
  • ATG10
  • Autophagy-related protein 10
  • DKFZp586I0418
  • EC 6.3.2.-
  • FLJ13954
  • PP12616
  • ubiquitin-like-conjugating enzyme ATG10

Background

Atg10 is a major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG10 interacts with LC3A to facilitate the conjugation of ATG12 to ATG5. APG10 is also able to directly interact either with ATG5 or ATG7A. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATG10 Antibody (NBP3-16649) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG10 Antibody (NBP3-16649) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ATG10 Products

Research Areas for ATG10 Antibody (NBP3-16649)

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Blogs on ATG10.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

From Then ‘till Now: The History of Autophagy and Cancer Research
By Christina Towers, PhD. The fundamental process that cells use to degrade damaged cytoplasmic material and recycle nutrients is called autophagy.  This term was first coined by the Belgium biochemist Christian de...  Read full blog post.

ATG12 - a ubiquitin-like protein essential for autophagosome assembly
Atg12 is a ubiquitin-like protein that plays an essential role in cellular homeostasis by regulating the degradation and recycling of cytoplasmic organelles and macromolecules. Atg12 is one of two ubiquitin-like protein systems that is required du...  Read full blog post.

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Bioinformatics

Gene Symbol ATG10