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ASAH1 Antibody (2C9)

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Immunohistochemistry-Paraffin: ASAH1 Antibody (2C9) [H00000427-M01] - Analysis of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Sandwich ELISA: ASAH1 Antibody (2C9) [H00000427-M01] - Detection limit for recombinant GST tagged ASAH1 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
2C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ASAH1 Antibody (2C9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ASAH1 (NP_808592, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKVMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTD
Specificity
ASAH1 - N-acylsphingosine amidohydrolase (acid ceramidase) 1
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ASAH1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Publications
Read Publications using H00000427-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASAH1 Antibody (2C9)

  • ACDase
  • ACEC 3.5.1.23
  • Acid CDase
  • acid ceramidase
  • Acylsphingosine deacylase
  • ASAH
  • FLJ21558
  • FLJ22079
  • N-acylsphingosine amidohydrolase (acid ceramidase) 1
  • N-acylsphingosine amidohydrolase
  • PHP
  • PHP32N-acylsphingosine amidohydrolase (acid ceramidase)
  • Putative 32 kDa heart protein

Background

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASAH1 Antibody (H00000427-M01)(2)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ASAH1
Entrez
OMIM
Uniprot