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Aromatase Antibody

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Western Blot: Aromatase Antibody [NB100-93375] - Analysis of Aromatase using this antibody at 0.1 ug/ml in Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Aromatase Antibody Summary

Immunogen
Peptide with sequence C-HDLSLHPDETKN corresponding to internal region according to NP_000094.2, NP_112503.1.
Epitope
C-HDLSLHPDETKN
Specificity
This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYP19A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 50-55 kDa band observed in human placenta lysates (calculated MW of 57.9 kDa band according to NP_000094.2 and NP_112503.1).
Control
Aromatase Overexpression Lysate
Publications
Read Publication using NB100-93375.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aromatase Antibody

  • ARO1
  • ARO1EC 1.14.14.1
  • Aromatase
  • AROsubfamily XIX (aromatization of androgens)
  • CYAR
  • CYARMGC104309
  • CYP19
  • CYP19A1
  • CYPXIX
  • cytochrome P450, family 19, subfamily A, polypeptide 1
  • Cytochrome P-450AROM
  • EC 1.14.14.14
  • Estrogen synthase
  • microsomal monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aromatase Antibody (NB100-93375)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Aromatase Products

Research Areas for Aromatase Antibody (NB100-93375)

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Blogs on Aromatase.

Aromatase - A Key Enzyme in the Biosynthesis of Estrogens
The enzyme, aromatase, belongs to cytochrome P450 family of monooxygenases known for their key role in drug catabolism and cholesterol/steroid synthesis. Aromatase uses a heme-group as a co-factor to catalyze the formation of aromatic C18 estrogens fr...  Read full blog post.

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Bioinformatics

Gene Symbol CYP19A1
Entrez
Uniprot