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ARF5 Antibody

Images

 
Western Blot: ARF5 Antibody [NBP1-31005] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF5 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: ARF5 Antibody [NBP1-31005] - Analysis of methanol-fixed HeLa, using ARF5 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ARF5 Antibody [NBP1-31005] - Paraffin-embedded human breast carcinoma. ARF5 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ARF5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary.
Localization
Golgi apparatus
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARF5
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for ARF5 Antibody

  • ADP-ribosylation factor 5

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ARF5 Antibody (NBP1-31005). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each protein that won't cross react with the other protein?
    • I checked the sequence similarity between ARF4 and ARF5, and there is very high homology between the two proteins. We have not specifically tested our antibodies for cross-reactivity between the two, but I think it's likely that they will.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF5
Entrez
Uniprot