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Aquaporin 1/AQP1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Analysis in human kidney and liver tissues. Corresponding AQP1 RNA-seq data are presented for the same tissues.
Western Blot: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Analysis in control (vector only transfected HEK293T lysate) and aQP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Aquaporin 1/AQP1 used at 1:50 on a feline cornea tissue. Aquaporin 1/AQP1 antibody was used on feline paraffin-embedded cornea tissue at a ...read more
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human lung shows strong membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human kidney shows moderate to strong positivity in apical membranes in cells in tubules.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human rectum shows moderate positivity in apical membranes in glandular cells.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, Fe, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Aquaporin 1/AQP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK
Predicted Species
Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Aquaporin 1/AQP1 Protein (NBP1-84488PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-84488 in the following applications:

Publications
Read Publications using
NBP1-84488 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30322799). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Aquaporin 1/AQP1 Antibody

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Background

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aquaporin 1/AQP1 Antibody (NBP1-84488)(2)

Review for Aquaporin 1/AQP1 Antibody (NBP1-84488) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NBP1-84488:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Feline
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Feline 05/11/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedcorneal stromal cells
SpeciesFeline
Lot000021263

Comments

CommentsAQP1 used at 1:50 on a feline cornea tissue. AQP1 antibody was used on feline paraffin-embedded cornea tissue at a concentration of 20ug/mL and left at 4C overnight. HIER was performed in Tris/EDTA buffer for two hours at 75C.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin 1/AQP1 Antibody (NBP1-84488). (Showing 1 - 1 of 1 FAQs).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Aquaporin 1/AQP1 Antibody (NBP1-84488)

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Blogs on Aquaporin 1/AQP1

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
05/11/2022
Application: IHC-P
Species: Feline

Bioinformatics

Gene Symbol AQP1