Orthogonal Strategies: Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Analysis in human kidney and liver tissues. Corresponding AQP1 RNA-seq data are presented for the same tissues.
Western Blot: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Analysis in control (vector only transfected HEK293T lysate) and aQP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Aquaporin 1/AQP1 used at 1:50 on a feline cornea tissue. Aquaporin 1/AQP1 antibody was used on feline paraffin-embedded cornea tissue at a ...read more
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human lung shows strong membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human kidney shows moderate to strong positivity in apical membranes in cells in tubules.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human rectum shows moderate positivity in apical membranes in glandular cells.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody [NBP1-84488] - Staining of human liver shows no positivity in hepatocytes as expected.
Mouse reactivity reported in scientific literature (PMID: 30322799). Feline reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Aquaporin 1/AQP1 Antibody
28-kDa
AQP1
AQP-1
AQP-CHIP
aquaporin 1 (channel-forming integral protein, 28kDa)
aquaporin 1 (Colton blood group)
Aquaporin 1
Aquaporin-CHIP
CHIP28
CHIP2828kDa, CO blood group)
CO
MGC26324
Background
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Review for Aquaporin 1/AQP1 Antibody (NBP1-84488) (1)
51
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Feline.
Reviews using NBP1-84488:
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Date
Details
5
reviewed by: Verified Customer
IHC-P
Feline
05/11/2022
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Summary
Application
Immunohistochemistry-Paraffin
Sample Tested
corneal stromal cells
Species
Feline
Lot
000021263
Comments
Comments
AQP1 used at 1:50 on a feline cornea tissue. AQP1 antibody was used on feline paraffin-embedded cornea tissue at a concentration of 20ug/mL and left at 4C overnight. HIER was performed in Tris/EDTA buffer for two hours at 75C.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Aquaporin 1/AQP1 Antibody (NBP1-84488). (Showing 1 - 1 of 1 FAQs).
Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.
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