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Aquaporin 1/AQP1 Antibody (S03-7G5)

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Western Blot: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572] - Western blot detection of Aquaporin 1/AQP1 in C6 cell lysates using NBP3-19572 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572] - Immunohistochemistry of Aquaporin 1/AQP1 in paraffin-embedded Human breast cancer tissue using NBP3-19572 at dilution 1/100

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
S03-7G5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

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Aquaporin 1/AQP1 Antibody (S03-7G5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Aquaporin 1/AQP1 (Uniprot # P29972)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
AQP1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/50-1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/2000
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for Aquaporin 1/AQP1 Antibody (S03-7G5)

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Background

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Aquaporin 1/AQP1 Antibody (NBP3-19572). (Showing 1 - 1 of 1 FAQ).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP1