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Apolipoprotein B/ApoB Antibody (APOB/3300) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (APOB/3300) [NBP3-08226] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein B/ApoB Mouse Monoclonal Antibody (APOB/3300).
Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody (APOB/3300) [NBP3-08226] - Formalin-fixed, paraffin-embedded human liver stained with Apolipoprotein B/ApoB Mouse Monoclonal Antibody (APOB/3300).
Protein Array: Apolipoprotein B/ApoB Antibody (APOB/3300) [NBP3-08226] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Apolipoprotein B/ApoB Mouse Monoclonal Antibody (APOB/3300).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
APOB/3300
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Apolipoprotein B/ApoB Antibody (APOB/3300) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07230).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Human recombinant Apolipoprotein B/ApoB protein fragment (around aa592-689) (exact sequence is proprietary) (Uniprot: P04114)
Localization
Cytoplasm, Secreted
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
512 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Apolipoprotein B/ApoB Antibody (APOB/3300) - Azide and BSA Free

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOB