![Western Blot: APOBEC2 Antibody [NB100-93372] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/APOBEC2-Antibody-Western-Blot-NB100-93372-img0002.jpg "Western Blot: APOBEC2 Antibody [NB100-93372] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EAASQNGEDLENLDD corresponding to internal region (near N-Terminus) according to NP_006780.1. |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOBEC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 26 kDa band observed in human heart and skeletal muscle lysates (calculated MW of 25.7 kDa band according to NP_006780.1).
An additional band of 90 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB. |
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| Theoretical MW | 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-93372 | Applications | Species |
|---|---|---|
| Prochnow C, Bransteitter R, Klein MG et al. The APOBEC-2 crystal structure and functional implications for the deaminase AID. Nature 2007-01-25 [PMID: 17187054] |
Secondary Antibodies |
Isotype Controls |
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