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AP1S1 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

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AP1S1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human AP1S1.

Source: E. coli

Amino Acid Sequence: KKSVLKAIEQADLLQEEDESPRSVLEEMGLA

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
AP1S1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10-100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-46862.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for AP1S1 Recombinant Protein Antigen

  • adaptor-related protein complex 1, sigma 1 subunit
  • AP19HA1 19 kDa subunit
  • CLAPS1FLJ92436
  • Clathrin assembly protein complex 1 sigma-1A small chain
  • Clathrin coat assembly protein AP19
  • Golgi adaptor HA1/AP1 adaptin sigma-1A subunit
  • Sigma 1a subunit of AP-1 clathrin
  • sigma1A subunit of AP-1 clathrin adaptor complex
  • SIGMA1A
  • sigma1A-adaptin
  • Sigma-adaptin 1A
  • small 1 (19kD)

Background

The protein encoded by the AP1S1 gene is part of the clathrin coat assembly complex which links clathrin to receptors incoated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well asbeta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located atthe Golgi vesicle. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol AP1S1