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Angiotensin II ELISA Kit (Colorimetric)

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ELISA: Non-species specific Angiotensin II ELISA Kit (Colorimetric) [NBP2-62135] - Pooled human plasma was spiked with 500 pg/mL of Angiotensin II, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was processed identically as a background control.
ELISA: Non-species specific Angiotensin II ELISA Kit (Colorimetric) [NBP2-62135] - Pooled human plasma was spiked with 500 pg/mL of Angiotensin II, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was ...read more
ELISA: Non-species specific Angiotensin II ELISA Kit (Colorimetric) [NBP2-62135] - A typical standard curve for Angiotensin II ELISA kit. These standard curves are provided for demonstration only. A standard curve ...read more

Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma,serum
Standard Curve Range
3.9-10,000 pg/ml
Sensitivity
4.6 pg/ml

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Angiotensin II ELISA Kit (Colorimetric) Summary

Specificity
Crossreactivity: Angiotensin II (100%), Angiotensin A (100%), Angiotensin III (100%), Angiotensin IV (100%), Angiotensin I (0.319%), Angiotensin (1-9) (0.103%), LVV-hemorphin 7 (0.067%), Angiotensin (1-7) (0.053%), Bradykinin (0.022%)
Standard Curve Range
3.9-10,000 pg/ml
Sensitivity
4.6 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
Low Concentration %CV - 6.0% Medium Concentration %CV - 7.5% High Concentration %CV - 15.9%
Intra-Assay
Low Concentration %CV - 7.3% Medium Concentration %CV - 5.2% High Concentration %CV - 4.7%
Spike Recovery
Low Concentration %CV - 80.0%
Medium - 69.0%
High - 52.2%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AGT

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-62135.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Antibody
  2. Assay Buffer 16
  3. Conjugate
  4. Microtiter Plate
  5. Standard
  6. Stop Solution 2
  7. Streptavidin-HRP
  8. TMB Substrate
  9. Wash Buffer Concentrate

Alternate Names for Angiotensin II ELISA Kit (Colorimetric)

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • angiotensin I
  • ANHU
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AGT