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Angiotensin I ELISA Kit (Colorimetric)

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ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - Pooled human plasma was spiked with 500pg/ml of Angiotensin I, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was processed identically as a background control.
ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - Pooled human plasma was spiked with 500pg/ml of Angiotensin I, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was ...read more
ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - A typical standard curve for Angiotensin I ELISA kit. These standard curves are provided for demonstration only. A standard curve should ...read more

Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma,serum
Standard Curve Range
3.9-10,000 pg/ml
Sensitivity
4.3 pg/ml

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Angiotensin I ELISA Kit (Colorimetric) Summary

Specificity
Crossreactivity: Angiotensin I (100%), Angiotensin A (0.182%), Angiotensin II (0.083%), Angiotensin (1-12) (0.025%). Unaffected by Angiotensin (1-9), Angiotensin (1-7), Angiotensin III, Angiotensin IV, LVV-hemorphin 7, Bradykinin.
Standard Curve Range
3.9-10,000 pg/ml
Sensitivity
4.3 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
Low Concentration %CV - 7.6% Medium Concentration %CV - 10.0% High Concentration %CV - 7.6%
Intra-Assay
Low Concentration %CV - 10.0% Medium Concentration %CV - 7.3% High Concentration %CV - 8.3%
Spike Recovery
Low Concentration %CV - 76.0%
Medium - 90.0%
High - 66.0%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AGT

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using NBP2-62134.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Antibody
  2. Assay Buffer 16
  3. Conjugate
  4. Microtiter Plate
  5. Standard
  6. Stop Solution 2
  7. Streptavidin-HRP
  8. TMB Substrate
  9. Wash Buffer Concentrate

Alternate Names for Angiotensin I ELISA Kit (Colorimetric)

  • alpha-1 antiproteinase, antitrypsin
  • angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • angiotensinogen
  • ANHU
  • FLJ92595
  • FLJ97926
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

The protein encoded by the Angiotensin I gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Angiotensin I ELISA Kit (NBP2-62134)(2)

We have publications tested in 1 application: ELISA.


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Bioinformatics

Gene Symbol AGT