![Western Blot: Aminopeptidase N/CD13 Antibody [NBP2-77451] - Total protein from human mouse and rat small intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD13 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-Western-Blot-NBP2-77451-img0002.jpg "Western Blot: Aminopeptidase N/CD13 Antibody [NBP2-77451] - Total protein from human mouse and rat small intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CD13 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant mouse CD13 protein (amino acids 69-966) [UniProt P97449] |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANPEP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]](https://images.novusbio.com/images2/Myeloperoxidase_AF3667_Western_Blot_5321.jpg)
![Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]](https://images.novusbio.com/images2/Myeloperoxidase_AF3667_Simple_Western_16637.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for Aminopeptidase N/CD13 Antibody (NBP2-77451)Find related products by research area.
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| Gene Symbol | ANPEP |
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-77451] - IHC analysis of a formalin fixed paraffin embedded tissue section of human prostate cancer using CD13 antibody at 1:50 dilution. The signal was developed with HRP-DAB detection method and the nuclei were counterstained with hematoxylin staining. This CD13 antibody generated a specific membrane staining in the cancer cells and the staining was localized more towards the secretory surface of the glands.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-Immunohistochemistry-Paraffin-NBP2-77451-img0001.jpg "Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody [NBP2-77451] - IHC analysis of a formalin fixed paraffin embedded tissue section of human prostate cancer using CD13 antibody at 1:50 dilution. The signal was developed with HRP-DAB detection method and the nuclei were counterstained with hematoxylin staining. This CD13 antibody generated a specific membrane staining in the cancer cells and the staining was localized more towards the secretory surface of the glands.")
![Flow Cytometry: Aminopeptidase N/CD13 Antibody [NBP2-77451] - A surface stain was performed on THP-1 cells with CD13 Antibody NBP2-77451 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-Flow-Cytometry-NBP2-77451-img0003.jpg "Flow Cytometry: Aminopeptidase N/CD13 Antibody [NBP2-77451] - A surface stain was performed on THP-1 cells with CD13 Antibody NBP2-77451 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")