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AlphaB Crystallin/CRYAB Antibody (CL10765)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB ...read more
Western Blot: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL10765
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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AlphaB Crystallin/CRYAB Antibody (CL10765) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: NGPRKQVSGPERTIPITREE
Predicted Species
Mouse (100%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CRYAB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (CL10765)

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Background

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CRYAB