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Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

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ELISA: Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) [NBP2-68200] - Samples were spiked with high concentrations of Rat alpha-Methylacyl-CoA Racemase/AMACR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) [NBP2-68200] - Samples were spiked with high concentrations of Rat alpha-Methylacyl-CoA Racemase/AMACR and diluted with Reference Standard & Sample ...read more
ELISA: Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) [NBP2-68200] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL

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Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat AMACR in samples. No significant cross-reactivity or interference between Rat AMACR and analogues was observed.
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.79%
Intra-Assay
CV% < 5.13%
Spike Recovery
87-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AMACR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric)

  • 2-methylacyl-CoA racemase
  • alpha-Methylacyl-CoA Racemase
  • alphaMethylacylCoARacemase
  • AMACR
  • AMACRD
  • CBAS4
  • EC 5.1.99.4
  • RACE
  • RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat alpha-Methylacyl-CoA Racemase/AMACR ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol AMACR