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alpha-Defensin 1 Antibody

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Western Blot: alpha-Defensin 1 Antibody [NBP2-27562] - Analysis of Human Spleen lysate (35ug protein in RIPA buffer) using NBP2-27562 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: alpha-Defensin 1 Antibody [NBP2-27562] - Analysis of Human Spleen lysate (35ug protein in RIPA buffer) using NBP2-27562 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: alpha-Defensin 1 Antibody [NBP2-27562] - (3.8 ug/ml) Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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alpha-Defensin 1 Antibody Summary

Immunogen
Peptide with sequence C-DESLAPKHPGSRKN corresponding to internal region according to NP_004075.1, NP_005208.1.
Specificity
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DEFA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa band according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Thymus shows staining of selected cells.
Publications
Read Publication using NBP2-27562.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Defensin 1 Antibody

  • alpha 2
  • alphaDefensin 1
  • alpha-Defensin 1
  • DEF1
  • DEF1DEFA1B
  • DEFA1
  • DEFA2
  • defensin, alpha 1, myeloid-related sequence
  • defensin, alpha 1HP-1
  • HNP-1
  • HNP-1HP1
  • HP1
  • MRS
  • MRSMGC138393
  • myeloid-related sequence
  • neutrophil defensin 1

Background

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundantin the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine,respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequenceand distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8.The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likelyplays a role in phagocyte-mediated host defense. It differs from defensin, alpha 3 by only one amino acid. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Defensin 1 Antibody (NBP2-27562)(1)


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Bioinformatics

Gene Symbol DEFA1
Uniprot