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alpha-Defensin 1 Antibody [Unconjugated]

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a-Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
a-Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human a-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight ...read more
Recombinant Human alpha ‑Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha ‑Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF8198-SP
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alpha-Defensin 1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human alpha ‑Defensin 1
Ala65-Cys94
Accession # P59665
Specificity
Detects human  alpha ‑Defensin 1 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DEFA1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF8198 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Defensin 1 Antibody [Unconjugated]

  • alpha 2
  • alphaDefensin 1
  • alpha-Defensin 1
  • DEF1
  • DEF1DEFA1B
  • DEFA1
  • DEFA2
  • defensin, alpha 1, myeloid-related sequence
  • defensin, alpha 1HP-1
  • HNP-1
  • HNP-1HP1
  • HP1
  • MRS
  • MRSMGC138393
  • myeloid-related sequence
  • neutrophil defensin 1

Background

Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 ( aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production. Phosphorylation at Tyr-85 is reported in several cancer cell lines and interferes with ADP-ribosylation of DEFA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Defensin 1 Antibody (AF8198)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol DEFA1
Uniprot