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Human Alpha Actinin 2 ELISA Kit (Chemiluminescence)

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ELISA: Human Alpha Actinin 2 ELISA Kit (Chemiluminescence) [NBP2-66417] - Samples were spiked with high concentrations of Human Alpha Actinin 2 and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Human Alpha Actinin 2 ELISA Kit (Chemiluminescence) [NBP2-66417] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

Human Alpha Actinin 2 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
Standard Curve Range
78.13-5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.17%
Intra-Assay
CV% < 9.78%
Spike Recovery
84-111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
ACTN2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Alpha Actinin 2 ELISA Kit (Chemiluminescence)

  • Actinin alpha 2
  • actinin, alpha 2
  • Actinin-a2
  • ACTN2
  • alpha Actinin 2
  • alpha-Actinin 2
  • Alpha-actinin skeletal muscle isoform 2
  • Alpha-Actinin Skeletal Muscle
  • Alpha-Actinin-2
  • CMD1AA
  • F-actin cross-linking protein

Background

Alpha actinin 2 is an actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ACTN2