I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

Name: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80568
Price: 399 USD

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Accepted Answer

None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, Flow, Func, ICC/IF, IHC
Clone	8B6
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	Azide and BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB110-3638AF350

Alexa Fluor 405 NB110-3638AF405

Alexa Fluor 488 NB110-3638AF488

Alexa Fluor 532 NB110-3638AF532

Alexa Fluor 594 NB110-3638AF594

Alexa Fluor 647 NB110-3638AF647

Alexa Fluor 700 NB110-3638AF700

Alexa Fluor 750 NB110-3638AF750

Allophycocyanin NB110-3638APC

Allophycocyanin/Cy7 NB110-3638APCCY7

Biotin NB110-3638B

DyLight 350 NB110-3638UV

DyLight 405 NB110-3638V

DyLight 488 NB110-3638G

DyLight 550 NB110-3638R

DyLight 594 NB110-3638DL594

DyLight 650 NB110-3638C

DyLight 680 NB110-3638FR

DyLight 755 NB110-3638IR

FITC NB110-3638F

HRP NB110-3638H

Janelia Fluor 549 NB110-3638JF549

Janelia Fluor 646 NB110-3638JF646

PE NB110-3638PE

PE/Atto594 NB110-3638PEATT594

PE/Cy5.5 NB110-3638PECY55

PE/Cy7 NB110-3638PECY7

PerCP NB110-3638PCP

Unconjugated NB110-3638

mFluor Violet 450 SE NB110-3638MFV450

mFluor Violet 500 SE NB110-3638MFV500

mFluor Violet 610 SE NB110-3638MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB110-3638

BSA Free NB110-3638

BSA Free NB110-3638SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-3638AF350
Alexa Fluor 405	NB110-3638AF405
Alexa Fluor 488	NB110-3638AF488
Alexa Fluor 532	NB110-3638AF532
Alexa Fluor 594	NB110-3638AF594
Alexa Fluor 647	NB110-3638AF647
Alexa Fluor 700	NB110-3638AF700
Alexa Fluor 750	NB110-3638AF750
Allophycocyanin	NB110-3638APC
Allophycocyanin/Cy7	NB110-3638APCCY7
Biotin	NB110-3638B
DyLight 350	NB110-3638UV
DyLight 405	NB110-3638V
DyLight 488	NB110-3638G
DyLight 550	NB110-3638R
DyLight 594	NB110-3638DL594
DyLight 650	NB110-3638C
DyLight 680	NB110-3638FR
DyLight 755	NB110-3638IR
FITC	NB110-3638F
HRP	NB110-3638H
Janelia Fluor 549	NB110-3638JF549
Janelia Fluor 646	NB110-3638JF646
PE	NB110-3638PE
PE/Atto594	NB110-3638PEATT594
PE/Cy5.5	NB110-3638PECY55
PE/Cy7	NB110-3638PECY7
PerCP	NB110-3638PCP
Unconjugated	NB110-3638
mFluor Violet 450 SE	NB110-3638MFV450
mFluor Violet 500 SE	NB110-3638MFV500
mFluor Violet 610 SE	NB110-3638MFV610

Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free

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Immunogen	Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Localization	Cell membrane; Lipid-anchor > GPI-anchor
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	ALPP
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	ELISA 1:100-1:2000 Flow Cytometry reported in multiple pieces of scientific literature Immunocytochemistry/ Immunofluorescence 1:10-1:500 Immunohistochemistry 1:10-1:500 Immunohistochemistry-Frozen 1:10-1:500 Immunohistochemistry-Paraffin 1:100-1:200 Radioimmunodiffusion Western Blot 1:1000
Application Notes	In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW	70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Tris-Glycine, 0.15 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Purity	Protein A purified