I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?

Name: Alkaline Phosphatase/ALPP Antibody (8B6) [mFluor Violet 450 SE]
Brand: Novus Biologicals
SKU: NB110-3638MFV450
Price: 519 USD

Question

Accepted Answer

None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, Flow, Func, ICC/IF, IHC
Clone	8B6
Clonality	Monoclonal
Host	Mouse
Conjugate	mFluor Violet 450 SE

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB110-3638AF350

Alexa Fluor 405 NB110-3638AF405

Alexa Fluor 488 NB110-3638AF488

Alexa Fluor 532 NB110-3638AF532

Alexa Fluor 594 NB110-3638AF594

Alexa Fluor 647 NB110-3638AF647

Alexa Fluor 700 NB110-3638AF700

Alexa Fluor 750 NB110-3638AF750

Allophycocyanin NB110-3638APC

Allophycocyanin/Cy7 NB110-3638APCCY7

Biotin NB110-3638B

DyLight 350 NB110-3638UV

DyLight 405 NB110-3638V

DyLight 488 NB110-3638G

DyLight 550 NB110-3638R

DyLight 594 NB110-3638DL594

DyLight 650 NB110-3638C

DyLight 680 NB110-3638FR

DyLight 755 NB110-3638IR

FITC NB110-3638F

HRP NB110-3638H

Janelia Fluor 549 NB110-3638JF549

Janelia Fluor 646 NB110-3638JF646

PE NB110-3638PE

PE/Atto594 NB110-3638PEATT594

PE/Cy5.5 NB110-3638PECY55

PE/Cy7 NB110-3638PECY7

PerCP NB110-3638PCP

Unconjugated NB110-3638

mFluor Violet 500 SE NB110-3638MFV500

mFluor Violet 610 SE NB110-3638MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB110-3638

BSA Free NB110-3638

BSA Free NB110-3638SS

Azide and BSA Free NBP2-80568

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-3638AF350
Alexa Fluor 405	NB110-3638AF405
Alexa Fluor 488	NB110-3638AF488
Alexa Fluor 532	NB110-3638AF532
Alexa Fluor 594	NB110-3638AF594
Alexa Fluor 647	NB110-3638AF647
Alexa Fluor 700	NB110-3638AF700
Alexa Fluor 750	NB110-3638AF750
Allophycocyanin	NB110-3638APC
Allophycocyanin/Cy7	NB110-3638APCCY7
Biotin	NB110-3638B
DyLight 350	NB110-3638UV
DyLight 405	NB110-3638V
DyLight 488	NB110-3638G
DyLight 550	NB110-3638R
DyLight 594	NB110-3638DL594
DyLight 650	NB110-3638C
DyLight 680	NB110-3638FR
DyLight 755	NB110-3638IR
FITC	NB110-3638F
HRP	NB110-3638H
Janelia Fluor 549	NB110-3638JF549
Janelia Fluor 646	NB110-3638JF646
PE	NB110-3638PE
PE/Atto594	NB110-3638PEATT594
PE/Cy5.5	NB110-3638PECY55
PE/Cy7	NB110-3638PECY7
PerCP	NB110-3638PCP
Unconjugated	NB110-3638
mFluor Violet 500 SE	NB110-3638MFV500
mFluor Violet 610 SE	NB110-3638MFV610

Alkaline Phosphatase/ALPP Antibody (8B6) [mFluor Violet 450 SE]

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Bioinformatics

Immunogen	Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Specificity	Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	ALPP
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	ELISA Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Frozen Immunohistochemistry-Paraffin Radioimmunodiffusion Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A purified