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Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

Images

 
BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by ...read more
Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 ...read more
Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more
Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc ...read more
iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
B4-78
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated] Summary

Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALPL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 3 Reviews rated 5
using
MAB1448 in the following applications:

Publications
Read Publications using
MAB1448 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALP
  • TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPL Antibody (MAB1448)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(6)
IHC-P
(1)
Immunohistochemistry
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
Rat
(2)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB1448 Applications Species
Anerillas, LO;Wiberg, M;Kingham, PJ;Kelk, P; Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs Regenerative therapy 2023-12-01 [PMID: 37588134] (Immunohistochemistry, Human) Immunohistochemistry Human
Mensali, N;Köksal, H;Joaquina, S;Wernhoff, P;Casey, NP;Romecin, P;Panisello, C;Rodriguez, R;Vimeux, L;Juzeniene, A;Myhre, MR;Fåne, A;Ramírez, CC;Maggadottir, SM;Duru, AD;Georgoudaki, AM;Grad, I;Maturana, AD;Gaudernack, G;Kvalheim, G;Carcaboso, AM;de Alava, E;Donnadieu, E;Bruland, ØS;Menendez, P;Inderberg, EM;Wälchli, S; ALPL-1 is a target for chimeric antigen receptor therapy in osteosarcoma Nature communications 2023-06-08 [PMID: 37291203] (ICC, Human) ICC Human
S Martin-Igl, L Milian, M Sancho-Tel, R Salvador-C, JJ Martín de, C Carda, M Mata BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models Stem Cells International, 2022-03-04;2022(0):4910399. 2022-03-04 [PMID: 35283997] (ICC, Human) ICC Human
Seiya Kanno, Yusuke Okubo, Tatsuto Kageyama, Lei Yan, Satoshi Kitajima, Junji Fukuda Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption iScience 2/18/2022 [PMID: 35146387]
Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al. miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition Molecular Therapy - Nucleic Acids 2021-03-05 [PMID: 33717654]
J Camps, H Grosemans, R Gijsbers, C Maes, M Sampaolesi Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells Int J Mol Sci, 2019-03-05;20(5):. 2019-03-05 [PMID: 30841538] (Flow Cytometry, Human, Mouse) Flow Cytometry Human, Mouse
Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS ONE, 2015-03-12;10(3):e0118499. 2015-03-12 [PMID: 25764185] (ICC, Human) ICC Human
Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy. J Immunol Res, 2014-04-30;2014(0):230346. 2014-04-30 [PMID: 24877156] (Flow Cytometry, Human) Flow Cytometry Human
A Nabeyama, K Nakano, S Saito, M sato, N Okafuji, A Yamamoto et al. Immunohistochemical expression of hard tissue related factors in the mouse dental pulp after immediate teeth separation European Journal of Medical Research 2011-11-10 [PMID: 22027645]
Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells. Bone, 2011-03-01;48(6):1417-26. 2011-03-01 [PMID: 21376148] (IHC-P, Rat) IHC-P Rat
Show All 15 Publications.

Reviews for Alkaline Phosphatase/ALPL Antibody (MAB1448) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using MAB1448:
Filter by Applications
WB
(1)
Flow
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 09/13/2022
View

Summary

ApplicationWestern Blot
Sample Testedengineered ALPL cells
SpeciesHuman
LotIKX0821121

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
  5
reviewed by:
Verified Customer
Flow Human 09/12/2022
View

Summary

ApplicationFlow Cytometry
Sample Testedengineered ALPL overexpressing cell line
SpeciesHuman
LotIKX0821121
  5
reviewed by:
Verified Customer
Flow Human 02/10/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 23514783
SpeciesHuman

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FAQs for Alkaline Phosphatase/ALPL Antibody (MAB1448) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
09/13/2022
Application: WB
Species: Human

Verified Customer
09/12/2022
Application: Flow
Species: Human

 
Verified Customer
02/10/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol ALPL