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Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin]

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Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, ICC/IF
Clone
B4-78
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1448U-100UG
Alexa Fluor 405
FAB1448V-100UG
Alexa Fluor 488
FAB1448G-100UG
Alexa Fluor 532
FAB1448X
Alexa Fluor 594
FAB1448T-100UG
Alexa Fluor 647
FAB1448R-100UG
Alexa Fluor 700
FAB1448N-100UG
Alexa Fluor 750
FAB1448S-100UG
Allophycocyanin
FAB1448A-100
Allophycocyanin
FAB1448A-025
Allophycocyanin/Cy7
FAB1448APCCY7
CoraFluor 1
FAB1448CL1
DyLight 350
FAB1448D
DyLight 405
FAB1448E
DyLight 488
FAB1448K
DyLight 550
FAB1448L
DyLight 594
FAB1448M
DyLight 650
FAB1448W
DyLight 680
FAB1448Y
DyLight 755
FAB1448Z
FITC
FAB1448F
HRP
FAB1448H
Janelia Fluor 525
FAB1448JF525
Janelia Fluor 549
FAB1448I
Janelia Fluor 646
FAB1448J
Janelia Fluor 669
FAB1448JF669
PE/Atto594
FAB1448PEATT594
PE/Cy5.5
FAB1448PECY55
PE/Cy7
FAB1448PECY7
Phycoerythrin
FAB1448P-025
Phycoerythrin
FAB1448P-100
Unconjugated
MAB1448
mFluor Violet 450 SE
FAB1448MFV450
mFluor Violet 500 SE
FAB1448MFV500
mFluor Violet 610 SE
FAB1448MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1448
Carrier Free
MAB1448
Carrier Free
MAB1448-SP
Carrier Free
FAB1448P-100
Carrier Free
FAB1448P-025
Carrier Free
FAB1448A-100
Carrier Free
FAB1448A-025
Carrier Free
FAB1448V-100UG
Carrier Free
FAB1448U-100UG
Carrier Free
FAB1448T-100UG
Carrier Free
FAB1448S-100UG
Carrier Free
FAB1448R-100UG
Carrier Free
FAB1448N-100UG
Carrier Free
FAB1448G-100UG
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Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin] Summary

Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALPL
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Readout System
Publications
Read Publications using
BAM1448 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALP
  • TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPL Antibody (BAM1448)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Cell Selection, Flow Cytometry, ICC.


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Cell Selection
(1)
Flow Cytometry
(1)
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(1)
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(3)
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Bioinformatics

Gene Symbol ALPL