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ALG8 Antibody

Images

 
Western Blot: ALG8 Antibody [NBP2-47550] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: ALG8 Antibody [NBP2-47550] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550] - Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ALG8 Antibody [NBP2-47550] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ALG8 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: AVRECCKCIDGKKVGKELTEKPKF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALG8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ALG8 Recombinant Protein Antigen (NBP2-47550PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ALG8 Antibody

  • alpha-1,3-glucosyltransferase)
  • asparagine-linked glycosylation 8 homolog (S. cerevisiae
  • asparagine-linked glycosylation 8 homolog (yeast
  • asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S.cerevisiae)
  • Asparagine-linked glycosylation protein 8 homolog
  • CDG1H
  • dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
  • Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
  • dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl-alpha-1,3-glucosyltransferase
  • EC 2.4.1
  • EC 2.4.1.-
  • HUSSY-02
  • MGC2840
  • probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Background

ALG8 - asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ALG8