Rat ALDH2 ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Rat ALDH2. No significant cross-reactivity or interference between Rat ALDH2 and analogues was observed. |
Standard Curve Range |
0.312 ng/mL - 20 ng/mL |
Sensitivity |
0.078 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Spike Recovery |
86 - 96% |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ALDH2 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate (12 x 8 coated Microwells)
- Biotin-antibody (100 x concentrate)
- Biotin-antibody Diluent
- HRP-avidin (100 x concentrate)
- HRP-avidin Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Rat ALDH2 ELISA Kit (Colorimetric)
Background
ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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