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Rat ALDH2 ELISA Kit (Colorimetric)

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Rat ALDH2 ELISA Kit (Colorimetric) [NBP2-77016] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
0.312 ng/mL - 20 ng/mL
Sensitivity
0.078 ng/mL

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Rat ALDH2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat ALDH2. No significant cross-reactivity or interference between Rat ALDH2 and analogues was observed.
Standard Curve Range
0.312 ng/mL - 20 ng/mL
Sensitivity
0.078 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
86 - 96%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ALDH2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay Plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction Manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Rat ALDH2 ELISA Kit (Colorimetric)

  • acetaldehyde dehydrogenase 2
  • aldehyde dehydrogenase 2 family (mitochondrial)
  • aldehyde dehydrogenase, mitochondrial
  • ALDH class 2
  • ALDH2
  • ALDH-E2
  • ALDHI
  • ALDM
  • EC 1.2.1
  • EC 1.2.1.3
  • liver mitochondrial ALDH
  • MGC1806
  • nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ALDH2