Reactivity | MuSpecies Glossary |
Applications | Flow |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Alexa Fluor 488 |
Immunogen | E. coli-derived recombinant mouse AHR Asn706-Ser805 (Thr758Ala) Accession # P30561 |
Specificity | Detects human and mouse AHR in Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | AHR |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Publications |
|
Storage | Protect from light. Do not freeze.
|
Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
Preservative | Sodium Azide |
AHR (Aryl-hydrocarbon receptor; also bHLHE76) is a 100-105 kDa member of the bHLH/PAS transcription factor family. It is widely expressed and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes. Mouse AHR precursor is 848 amino acids (aa) in length. It contains a nine aa prosegment, plus an 839 aa mature molecule that contains a DNA binding motif (aa 12-39), a bHLH region (aa 40-80), two PAS domains (aa 116-336) and one PAC segment that stabilizes the PAS domains (aa 342-383). There are multiple alleles for mouse AHR. One 95-97 kDa allele shows a premature truncation after Ser805, while a second 112 kDa allele shows a 41 aa substitution for aa 843-848. Over aa 706-805, mouse AHR shares 87% and 63% aa identity with rat and human AHR, respectively.
Publication using IC6697G | Applications | Species |
---|---|---|
Rothhammer V, Borucki DM, Tjon EC et al. Microglial control of astrocytes in response to microbial metabolites Nature 5/1/2018 [PMID: 29769726] |
Secondary Antibodies |
Isotype Controls |
AHR - A transcription factor regulating immunity and tumorigenesis The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w... Read full blog post. |
Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more... AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.