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A33/GPA33 Antibody (402104) [Phycoerythrin]

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HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 PE-conjugated Monoclonal Antibody (Catalog # FAB3080P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 PE-conjugated Monoclonal Antibody (Catalog # FAB3080P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). ...read more
Colo-205 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 PE-conjugated Monoclonal Antibody (Catalog # FAB3080P, filled histogram) or isotype control antibody (IC006P, open histogram). Staining ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
402104
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3080U-100UG
Alexa Fluor 405
FAB3080V-100UG
Alexa Fluor 488
FAB3080G
Alexa Fluor 532
FAB3080X
Alexa Fluor 594
FAB3080T-100UG
Alexa Fluor 647
FAB3080R-100UG
Alexa Fluor 700
FAB3080N-100UG
Alexa Fluor 750
FAB3080S-100UG
Allophycocyanin
FAB3080A
Allophycocyanin/Cy7
FAB3080APCCY7
Biotin
FAB3080B
CoraFluor 1
FAB3080CL1
DyLight 350
FAB3080D
DyLight 405
FAB3080E
DyLight 488
FAB3080K
DyLight 550
FAB3080L
DyLight 594
FAB3080M
DyLight 650
FAB3080W
DyLight 680
FAB3080Y
DyLight 755
FAB3080Z
FITC
FAB3080F
HRP
FAB3080H
Janelia Fluor 525
FAB3080JF525
Janelia Fluor 549
FAB3080I
Janelia Fluor 585
FAB3080JF585
Janelia Fluor 635
FAB3080JF635
Janelia Fluor 646
FAB3080J
Janelia Fluor 669
FAB3080JF669
PE/Atto594
FAB3080PEATT594
PE/Cy5.5
FAB3080PECY55
PE/Cy7
FAB3080PECY7
PerCP
FAB3080C
Unconjugated
MAB3080
mFluor Violet 450 SE
FAB3080MFV450
mFluor Violet 500 SE
FAB3080MFV500
mFluor Violet 610 SE
FAB3080MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3080
Carrier Free
MAB3080
Carrier Free
FAB3080G
Carrier Free
FAB3080A
Carrier Free
MAB3080-SP
Carrier Free
FAB3080V-100UG
Carrier Free
FAB3080U-100UG
Carrier Free
FAB3080T-100UG
Carrier Free
FAB3080S-100UG
Carrier Free
FAB3080R-100UG
Carrier Free
FAB3080N-100UG
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A33/GPA33 Antibody (402104) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Specificity
Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
GPA33
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A33/GPA33 Antibody (402104) [Phycoerythrin]

  • A33
  • cell surface A33 antigen
  • glycoprotein A33 (transmembrane)
  • Glycoprotein A33
  • GPA33
  • MGC129986
  • MGC129987
  • transmembrane glycoprotein A33

Background

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Isotype Controls

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Bioinformatics

Gene Symbol GPA33
Uniprot