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14-3-3 sigma/Stratifin Antibody [Unconjugated]

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Western blot shows lysates of A431 human epithelial carcinoma cell line and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 sigma was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of A431 human epithelial carcinoma cell line and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3s Antigen Affinity-purified Polyclonal Antibody ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 14-3-3s knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4424-SP
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14-3-3 sigma/Stratifin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human 14-3-3 sigma
Met1-Ser248
Accession # P31947
Specificity
Detects human, mouse, and rat 14-3-3 sigma in Western blots. Does not cross react with other recombinant human 14‑3‑3 isoforms.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4424 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 sigma/Stratifin Antibody [Unconjugated]

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta , epsilon , eta , gamma , tau / theta , sigma , and zeta /δ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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14-3-3 sigma/Stratifin Antibody [Unconjugated]
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Publications for 14-3-3 sigma/Stratifin Antibody (AF4424)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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(2)
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Bioinformatics

Gene Symbol SFN
Uniprot