Home » 14-3-3 sigma/Stratifin » 14-3-3 sigma/Stratifin Antibodies » 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) - Azide and BSA Free
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14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) - Azide and BSA Free

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Western Blot: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-08214] - Western Blot Analysis of Human PC-3 cell lysate using 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).
Western Blot: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-08214] - Western Blot Analysis of Human PC-3 cell lysate using 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-08214] - Formalin-fixed, paraffin-embedded human Skin stained with 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).
Protein Array: 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) [NBP3-08214] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 sigma/Stratifin Mouse Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
CPTC-SFN-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08214AF350
Alexa Fluor 405
NBP3-08214AF405
Alexa Fluor 488
NBP3-08214AF488
Alexa Fluor 532
NBP3-08214AF532
Alexa Fluor 594
NBP3-08214AF594
Alexa Fluor 647
NBP3-08214AF647
Alexa Fluor 700
NBP3-08214AF700
Alexa Fluor 750
NBP3-08214AF750
Allophycocyanin
NBP3-08214APC
Biotin
NBP3-08214B
CoraFluor 1
NBP3-08214CL1
DyLight 350
NBP3-08214UV
DyLight 405
NBP3-08214V
DyLight 488
NBP3-08214G
DyLight 550
NBP3-08214R
DyLight 594
NBP3-08214DL594
DyLight 650
NBP3-08214C
DyLight 680
NBP3-08214FR
DyLight 755
NBP3-08214IR
FITC
NBP3-08214F
HRP
NBP3-08214H
Janelia Fluor 525
NBP3-08214JF525
Janelia Fluor 549
NBP3-08214JF549
Janelia Fluor 646
NBP3-08214JF646
Janelia Fluor 669
NBP3-08214JF669
PE
NBP3-08214PE
PerCP
NBP3-08214PCP
Unconjugated
NBP3-07326-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07326-100ug
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14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07326).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length 14-3-3 sigma/Stratifin protein (Uniprot: P31947)
Localization
Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SFN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for 14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) - Azide and BSA Free

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Alternate names for 14-3-3 sigma include epithelial marker protein 1, SFN and YWHAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-08214
14-3-3 sigma/Stratifin Antibody (CPTC-SFN-2) - Azide and BSA Free
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Bioinformatics

Gene Symbol SFN