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CD133 Antibody (170411) [Unconjugated]

Images

 
BG01V human embryonic stem cells was stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (MAB003, open histogram), followed by ...read more
iPS cells were stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
170411
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD133 Antibody (170411) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490
Specificity
Detects human CD133.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PROM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB11331 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD133 Antibody (170411) [Unconjugated]

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD133 Antibody (MAB11331)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Capture, ELISA Detection, ICC, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Detection
(1)
ICC
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 4 of 4.
Publications using MAB11331 Applications Species
Shariatzadeh, M;Nagtzaam, NMA;van Vark-van der Zee, L;van Holten-Neelen, C;Verhoeven, AJM;Dehairs, J;Swinnen, JV;Leijten, F;Ten Berge, JC;Ciriano, JPM;Wong, KT;Mulder, M;Leenen, PJM;Dik, WA; Altered Functionality of Lipoprotein(a) Impacts on Angiogenesis in Diabetic Retinopathy Investigative ophthalmology & visual science 2023-05-01 [PMID: 37159194] (ICC, Human) ICC Human
M Zouari, D Aissaoui-Z, S Campuzano, R Barderas, N Srairi-Abi, JM Pingarrón, N Raouafi Multipurpose E-bioplatform targeting Kv channels in whole cancer cells and evaluating of their potential therapeutics Analytica chimica acta, 2022-09-15;1231(0):340397. 2022-09-15 [PMID: 36220288] (ELISA Capture, Human) ELISA Capture Human
R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy PLoS ONE, 2019-05-02;14(5):e0215990. 2019-05-02 [PMID: 31048929] (ELISA Detection, Human) ELISA Detection Human
GY Chiou, TW Yang, CC Huang, CY Tang, JY Yen, MC Tsai, HY Chen, N Fadhilah, CC Lin, YJ Jong Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells Sci Rep, 2017-05-19;7(1):2172. 2017-05-19 [PMID: 28526879] (Western Blot, Human) Western Blot Human

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FAQs for CD133 Antibody (MAB11331) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CD133 Products

Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1
Uniprot