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Argininosuccinate Synthase Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining in human kidney and lymph node tissues using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney, liver, lymph node and urinary bladder using Anti-ASS1 antibody NBP1-88867 (A) ...read more
Orthogonal Strategies: Western Blot: Argininosuccinate Synthase Antibody [NBP1-88867] - Analysis in human cell lines MCF-7 and U-251MG using anti-ASS1 antibody. Corresponding ASS1 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human skin shows strong cytoplasmic positivity in a subset of squamous epithelial cells.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node using Anti-ASS1 antibody NBP1-88867.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human lymph node shows no cytoplasmic positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-88867] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Argininosuccinate Synthase Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQS
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Argininosuccinate Synthase Protein (NBP1-88867PEP)
Publications
Read Publication using NBP1-88867.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Argininosuccinate Synthase Antibody

  • argininosuccinate synthase 1
  • argininosuccinate synthase
  • argininosuccinate synthetase 1
  • argininosuccinate synthetase
  • ASSCTLN1
  • citrulline-aspartate ligase
  • Citrulline--aspartate ligase
  • EC 6.3.4.5

Background

Argininosuccinate Synthase is encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of Argininosuccinate Synthase cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Argininosuccinate Synthase Antibody (NBP1-88867)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ASS1