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Nuclear Migration Pathway Bioinformatics

Nuclear migration is a phenomenon that occurs in neurons during brain development. Movement of the nucleus occurs during the proliferative and post-mitotic phases of development, and there are three separate kinds of movement of the nucleus. Interkinetic nuclear migration is when the nucleus moves back and forth between the apical and basal sides of the cell depending on its necessary location in the cell cycle. Proliferative nuclear migration is when the nucleus begins on the apical side of the cell, and transfers to the basal side after the M-phase for the remainder of the cell cycle. The nucleus can also help with cellular migration, in a method called nuclear translocation. Microtubules from the cytoskeleton surround the nucleus, and with the help of certain proteins, physically move the nucleus, which in turns signals the cell to begin its migration.

Top Research Reagents

We have 2941 products for the study of the Nuclear Migration Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NFkB p105/p50 Antibody (2J10D7) - BSA Free
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review

Mouse Monoclonal
Species Human, Rat, Rabbit
Applications WB, Flow, IHC

     1 Review

5 Publications
LIS1 Antibody
NBP1-87769
Immunohistochemistry-Paraffin: LIS1 Antibody [NBP1-87769] - Analysis in human testis and liver tissues using NBP1-87769 antibody. Corresponding PAFAH1B1 RNA-seq data are presented for the same tissues.Western Blot: LIS1 Antibody [NBP1-87769] - Analysis of LIS1 in caco-2 whole cell lysate using anti-LIS1 antibody. Image submitted by a verified customer review.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     2 Reviews

APOBEC2 Antibody
NBP1-88848
Immunohistochemistry-Paraffin: APOBEC2 Antibody [NBP1-88848] - Analysis in human skeletal muscle and liver tissues using NBP1-88848 antibody. Corresponding APOBEC2 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: APOBEC2 Antibody [NBP1-88848] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NUDC Antibody
NBP1-89517
Western Blot: NUDC Antibody [NBP1-89517] - Analysis using Anti-NUDC antibody NBP1-89517 (A) shows similar pattern to independent antibody NBP1-89510 (B).Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89517] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
ARFRP1 Antibody
NBP2-14306
Western Blot: ARFRP1 Antibody [NBP2-14306] - Analysis in control (vector only transfected HEK293T lysate) and ARFRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: ARFRP1 Antibody [NBP2-14306] - Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

ACTR1A Antibody
NBP2-15274
Western Blot: ACTR1A Antibody [NBP2-15274] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE, antibody diluted at 1:2000.Immunohistochemistry-Paraffin: ACTR1A Antibody [NBP2-15274] - Paraffin-embedded Lung ca, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

ERK1 Antibody (1E5) - BSA Free
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

1 Publication
p21/CIP1/CDKN1A Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Chimpanzee
Applications WB, Flow, ICC/IF

24 Publications
Thrombopoietin R/Tpo R Antibody [Unconjugated]
AF1016
<P align=left>Recombinant Human Thrombopoietin/Tpo (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombopoietin R/Tpo R at approximately 73 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Thrombopoietin R/Tpo R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1016) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Goat Polyclonal
Species Human
Applications WB, Simple Western, Neut

4 Publications
ERK2 Antibody [Unconjugated]
AF1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class= ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, KO

3 Publications
PITX2 Antibody [Unconjugated]
AF7388
PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Sheep Polyclonal
Species Human
Applications IHC

8 Publications
Angiopoietin-like Protein 1/ANGPTL1 Antibody [Unconjugated]
AF2204
Angiopoietin‑like Protein 1/ANGPTL1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human Angiopoietin‑like Protein 1/ANGPTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2204) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, IHC

1 Publication
COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]
PP-H7147-00
Reduction of COUP-TFII or nucleolin decreases RAR beta 2 transcription in MCF-7 cells.MCF-7 (A) and T47D (B) cells were transfected with control siRNA or an siRNA targeting nucleolin for 48 h. T47D cells were treated with EtOH or 1 µM atRA for 24 h. Q-PCR for nucleolin (NCL) and RARB2. Values are the average of triplicates. C, Western blot showing COUP-TFII and RAR beta 2 expression after transfection with siCOUP-TFII. Values are relative to beta -actin. MCF-7 were transfected with siControl or siCOUP-TFII for 48 h and treated with 1 µM atRA for 6 h. Q-PCR was also performed for RRIG1. P<0.001 * versus control or ** versus atRA. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0038278), licensed under a CC-BY license. Not internally tested by R&D Systems.Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(a) LNCaP and PC3 cells were individually treated with 50 nM of miR-101 and miR-27a mimic for 48 h and then invasion assays were performed for an additional 16 h (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group. (b) Representative western blot showed the levels of COUP-TFII, E-cadherin, vimentin and beta -actin in PC3 cells stably knocked down by COUP-TFII. (c) LNCaP and PC3 cells stably knocked-down of COUP-TFII were used to perform cell invasion assay. Invaded cells were stained and counted (lower panel) (n=3). Representative western blot showed the knockdown efficiency of COUP-TFII (upper panel). *: P<0.05 (two-sided Student's t-test) compared with control group. (d) PC3 cells carrying an inducible miR-101 in the absence and presence of doxycycline, and re-expression of COUP-TFII were used to perform invasion assays (n=3). Representative western blot showed the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and results are shown in the right panel. *: P<0.05 (two-sided Student's t-test) (e) 22RV-1 cells were treated with miR-101 inhibitor (antisense RNA) in conjunction with knockdown of COUP-TFII and used for invasion assays (n=3). Representative western blot show the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and result is shown in the right panel. *: P<0.05 (two-sided Student's t-test). (f) LNCaP cells containing a construct with inducible expression of COUP-TFII shRNA and constructs expressing with anti-miR-101 and anti-mir-27a were orthotopically injected into NOD-SCID mouse prostate. In addition these cells also contain a luciferase reporter to detect cancer cells. After the tumour size was bigger than 50 mm3, drinking water with or without doxycycline (1 mg ml−1) was given to the mice for 6 weeks to induce the COUP-TFII shRNA to repress COUP-TFII expression. Representative bioluminescence results show the status of tumour growth in different groups. (g) Quantification result of luminance from IVIS (control: n=5; anti-miR-101/27a(−): n=6; anti-miR-101/27a(+): n=7. *: P<0.05 (two-sided Student's t-test). (h) Immunohistochemical stain showed the metastatic tumour cells located in the mouse lymph node are positive for AR. Scale bar: 100 μM (low power); and 200 μM (high power). SCID, severe combined immunodeficient. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse Monoclonal
Species Human
Applications WB, IHC, IP

     3 Reviews

45 Publications
PLA2G7/PAF-AH/Lp-PLA2 [HRP]
DPLG70
N/A PLA2G7/PAF-AH/Lp-PLA2 [HRP]N/A PLA2G7/PAF-AH/Lp-PLA2 [HRP]


Species Human
Applications ELISA

     1 Review

14 Publications
Thrombopoietin/THPO [Unconjugated]
288-TPN/CF
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED<sub>50</sub> for this effect is 5-15 ng/mL.


Species Human
Applications BA

12 Publications
TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

811 Publications
Nesprin 2 Antibody
NBP2-42886
Western Blot: Nesprin 2 Antibody [NBP2-42886] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nesprin 2 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Immunohistochemistry-Paraffin: Nesprin 2 Antibody [NBP2-42886] - Rat brain. Nesprin 2 stained by Nesprin 2 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

PCNA Antibody (PC10)
NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     5 Reviews

45 Publications
Neutrophil Elastase/ELA2 [Biotin]
DY9167-05
N/A Neutrophil Elastase/ELA2 [Biotin]


Species Human
Applications ELISA

19 Publications