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pcna Products By Gene Name

38 Results for "pcna"

NB500-106

PCNA Antibody (PC10)

(5 Reviews)

(45 Publications)

0.1 ml

NB500-106

Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.

Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.

Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.

Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review.

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY license.

Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

$Chromatin Immunoprecipitation: PCNA Antibody (PC10) [NB500-106] - Events taking place during a transient telomere dysfunction. All strains were cdc13–1, incubated for 160 min at 36°C to induce telomere uncapping, followed by 90 min at 23°C to allow telomere re-capping. Nocodazole & BrdU were added to the cultures at time 0. (A) Dynamics of ssDNA loss in sub-telomeres. (B) BrdU incorporation in sub-telomeres, minus incorporation at ‘CEN’ (e.g. a centromere-proximal locus, in this case ERG26). (C–F) Dynamics of the protein association with sub-telomeres, measured for Rap1 & major DNA synthesis factors (indicated above each graph). ChIP (%) was calculated as the fraction of immunoprecipitated sub-telomeric DNA, minus the fraction precipitated at ‘CEN’ (G) Dynamics of ssDNA loss at YER188W. (H) BrdU incorporation at YER188W, minus incorporation at ‘CEN’. (I–L) The protein association with YER188W was analyzed as in C–F. (M) ssDNA at ‘CEN’. (N) BrdU incorporation at ‘CEN’. (O–R) Association of proteins with ‘CEN’. (S) Growth of serial dilution of wild-type (first row) & cdc13–1 cells with or without additional mutations (indicated on the left of each row) at temperatures indicated above each plate. The plate shown on the right was cycled three times between 4 h at 36°C (to accumulate ssDNA) & 4 h at 21°C (to re-synthesize DNA), followed by incubation at 21°C for another two days. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. (A–D) Immunofluorescence for PCNA (yellow) & DAPI staining (blue) on 30 μm-cryosections of E32–P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 μm. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. (E) Quantification of the VZ thickness of the E32–P1 tree shrew neocortex. (F) Quantification of the SVZ thickness of the E32–P1 tree shrew neocortex. (G) Quantification of the VZ & SVZ thickness of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of VZ & SVZ. (H) Quantification of the iSVZ & oSVZ thickness of the E37 tree shrew neocortex, expressed as percentage of the sum of iSVZ & oSVZ. (E–H) Data represent mean ± SD & were obtained from two consecutive sections of two brains each. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29725291), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, Ze

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-106AF350
Alexa Fluor 405	NB500-106AF405
Alexa Fluor 488	NB500-106AF488
Alexa Fluor 532	NB500-106AF532
Alexa Fluor 594	NB500-106AF594
Alexa Fluor 647	NB500-106AF647
Alexa Fluor 700	NB500-106AF700
Alexa Fluor 750	NB500-106AF750
Allophycocyanin	NB500-106APC
CoraFluor 1	NB500-106CL1
DyLight 350	NB500-106UV
DyLight 405	NB500-106V
DyLight 488	NB500-106G
DyLight 550	NB500-106R
DyLight 594	NB500-106DL594
DyLight 650	NB500-106C
DyLight 680	NB500-106FR
DyLight 755	NB500-106IR
FITC	NB500-106F
HRP	NB500-106H
Janelia Fluor 525	NB500-106JF525
Janelia Fluor 549	NB500-106JF549
Janelia Fluor 585	NB500-106JF585
Janelia Fluor 635	NB500-106JF635
Janelia Fluor 646	NB500-106JF646
Janelia Fluor 669	NB500-106JF669
PE	NB500-106PE
PerCP	NB500-106PCP
mFluor Violet 450 SE	NB500-106MFV450
mFluor Violet 500 SE	NB500-106MFV500
mFluor Violet 610 SE	NB500-106MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80905

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NBP1-32075

PCNA Antibody

(2 Publications)

0.1 ml

NBP1-32075

Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug PC-12 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PCNA protein stained by PCNA antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse liver. PCNA antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PCNA Antibody [NBP1-32075] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500.

Western Blot: PCNA Antibody [NBP1-32075] - A. 30 ug NIH-3T3 whole cell lysate/extract 12 % SDS-PAGEPCNA antibody dilution: 1:2500

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-32075] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Mouse testis. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human colon. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Human cervical carcinoma. PCNA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-32075] - Rat hind brain. PCNA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: PCNA Antibody [NBP1-32075] - Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 ug of PCBP2 antibody. Western blot analysis was performed using PCBP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: PCNA Antibody [NBP1-32075] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (NBP1-32075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Dr, SyHa, Po, Bv, RM, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Dr, SyHa, Po, Bv, RM, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NB100-456

PCNA Antibody

(7 Publications)

100 ul

NB100-456

Western Blot: PCNA Antibody [NB100-456] - Detection of Human and Mouse PCNA by Western Blot. Samples: Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody NB100-456 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

<b>Biological Strategies Validation. </b>Immunohistochemistry: PCNA Antibody [NB100-456] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- PCNA used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB

Flow Cytometry: PCNA Antibody [NB100-456] - Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X106 cells were stained with 0.03 ug anti-PCNA . Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody], and DNA stained with PI.

Western Blot: PCNA Antibody [NB100-456] - A431 whole cell lysates

<b>Biological Strategies Validation. </b>Western Blot: PCNA Antibody [NB100-456] - Analysis using the Biotin conjugate of NB100-456. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.

<b>Independent Antibodies Validation. </b>Immunoprecipitation: PCNA Antibody [NB100-456] - Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody NB100-456 used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using another a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). immunoprecipitatesd PCNA was blotted using NB100-456 (1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).

Reactivity:

Hu, Mu, Rt, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-456AF350
Alexa Fluor 405	NB100-456AF405
Alexa Fluor 488	NB100-456AF488
Alexa Fluor 532	NB100-456AF532
Alexa Fluor 594	NB100-456AF594
Alexa Fluor 647	NB100-456AF647
Alexa Fluor 700	NB100-456AF700
Alexa Fluor 750	NB100-456AF750
Allophycocyanin	NB100-456APC
Biotin	NB100-456B
CoraFluor 1	NB100-456CL1
DyLight 350	NB100-456UV
DyLight 405	NB100-456V
DyLight 488	NB100-456G
DyLight 550	NB100-456R
DyLight 594	NB100-456DL594
DyLight 650	NB100-456C
DyLight 680	NB100-456FR
DyLight 755	NB100-456IR
FITC	NB100-456F
HRP	NB100-456H
Janelia Fluor 525	NB100-456JF525
Janelia Fluor 549	NB100-456JF549
Janelia Fluor 585	NB100-456JF585
Janelia Fluor 635	NB100-456JF635
Janelia Fluor 646	NB100-456JF646
Janelia Fluor 669	NB100-456JF669
PE	NB100-456PE
PerCP	NB100-456PCP
mFluor Violet 450 SE	NB100-456MFV450
mFluor Violet 500 SE	NB100-456MFV500
mFluor Violet 610 SE	NB100-456MFV610

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NBP2-37414

PCNA Antibody (7H4F8) - BSA Free

(1 Review)

0.1 ml

NBP2-37414

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA antibody. Antibody dilution 1:500. Image from verified customer review.

Flow Cytometry: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against human PCNA recombinant protein. (Expected MW is 41.2 kDa)

Western Blot: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis using PCNA mAb against HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of cervical cancer tissues using PCNA mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: PCNA Antibody (7H4F8) [NBP2-37414] - Analysis of colon cancer tissues using PCNA mouse mAb with DAB staining.

ELISA: PCNA Antibody (7H4F8) [NBP2-37414] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37414-0.025ml

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NBP2-67390

PCNA Antibody (SY12-07)

(1 Publication)

100 ul

NBP2-67390

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on L6 cell lysates with Rabbit anti-PCNA antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 36 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Flow Cytometry: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCNA Antibody (SY12-07) [NBP2-67390] - Analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-89503

PCNA Antibody

(1 Publication)

0.1 ml

NBP1-89503

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Analysis in human lymph node and liver tissues using NBP1-89503 antibody. Corresponding PCNA RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] - Staining of human colon, liver, lymph node and testis using Anti-PCNA antibody NBP1-89503 (A) shows similar protein distribution across tissues to independent antibody NBP1-89504 (B).

Western Blot: PCNA Antibody [NBP1-89503] - Analysis in human cell line MOLT-4.

Immunocytochemistry/Immunofluorescence: PCNA Antibody [NBP1-89503] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.

Western Blot: PCNA Antibody [NBP1-89503] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] -Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] -Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] -Staining of human testis shows strong nuclear positivity in seminiferous ducts.

Immunohistochemistry-Paraffin: PCNA Antibody [NBP1-89503] -Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-46342

PCNA Antibody (OTI3D6)

0.1 ml

NBP2-46342

Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA.

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: PCNA Antibody (3D6) [NBP2-46342] - Analysis of extracts (35ug) from 9 different cell lines by using PCNA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:2000

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PCNA Antibody (3D6) [NBP2-46342] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73271AF350
Alexa Fluor 405	NBP2-73271AF405
Alexa Fluor 488	NBP2-73271AF488
Alexa Fluor 532	NBP2-73271AF532
Alexa Fluor 594	NBP2-73271AF594
Alexa Fluor 647	NBP2-73271AF647
Alexa Fluor 700	NBP2-73271AF700
Alexa Fluor 750	NBP2-73271AF750
Allophycocyanin	NBP2-73271APC
Biotin	NBP2-73271B
CoraFluor 1	NBP2-73271CL1
DyLight 350	NBP2-73271UV
DyLight 405	NBP2-73271V
DyLight 488	NBP2-73271G
DyLight 550	NBP2-73271R
DyLight 594	NBP2-73271DL594
DyLight 650	NBP2-73271C
DyLight 680	NBP2-73271FR
DyLight 755	NBP2-73271IR
FITC	NBP2-73271F
HRP	NBP2-73271H
Janelia Fluor 525	NBP2-73271JF525
Janelia Fluor 549	NBP2-73271JF549
Janelia Fluor 585	NBP2-73271JF585
Janelia Fluor 635	NBP2-73271JF635
Janelia Fluor 646	NBP2-73271JF646
Janelia Fluor 669	NBP2-73271JF669
PE	NBP2-73271PE
PerCP	NBP2-73271PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73271

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NBP2-90058

PCNA Antibody (144)

(6 Publications)

100 ul

NBP2-90058

Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).

Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90058AF350
Alexa Fluor 405	NBP2-90058AF405
Alexa Fluor 488	NBP2-90058AF488
Alexa Fluor 532	NBP2-90058AF532
Alexa Fluor 594	NBP2-90058AF594
Alexa Fluor 647	NBP2-90058AF647
Alexa Fluor 700	NBP2-90058AF700
Alexa Fluor 750	NBP2-90058AF750
Allophycocyanin	NBP2-90058APC
Biotin	NBP2-90058B
CoraFluor 1	NBP2-90058CL1
DyLight 350	NBP2-90058UV
DyLight 405	NBP2-90058V
DyLight 488	NBP2-90058G
DyLight 550	NBP2-90058R
DyLight 594	NBP2-90058DL594
DyLight 650	NBP2-90058C
DyLight 680	NBP2-90058FR
DyLight 755	NBP2-90058IR
FITC	NBP2-90058F
HRP	NBP2-90058H
Janelia Fluor 525	NBP2-90058JF525
Janelia Fluor 549	NBP2-90058JF549
Janelia Fluor 585	NBP2-90058JF585
Janelia Fluor 635	NBP2-90058JF635
Janelia Fluor 646	NBP2-90058JF646
Janelia Fluor 669	NBP2-90058JF669
PE	NBP2-90058PE
PerCP	NBP2-90058PCP
mFluor Violet 450 SE	NBP2-90058MFV450
mFluor Violet 500 SE	NBP2-90058MFV500
mFluor Violet 610 SE	NBP2-90058MFV610

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NB100-478

PCNA Antibody (339)

(1 Publication)

100 ul

NB100-478

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000

Immunocytochemistry/Immunofluorescence: PCNA Antibody (339) [NB100-478] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using 1:500 dilution.

Western Blot: PCNA Antibody (339) [NB100-478]

Western Blot: PCNA Antibody (339) [NB100-478] - A: Jurkat , B: Raji, C: 293T, D: A431, E: HeLa, F: HepG2, G: H1299, H: HCT116, I: MCF-7, J: NT2D1, K: PC-3, L: U87-MG

Western Blot: PCNA Antibody (339) [NB100-478] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-32725

PCNA Antibody (A6-G11-R)

100 ul

NBP3-32725

Immunoprecipitation: PCNA Antibody (A6-G11-R) [NBP3-32725] - PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32725 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32725 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32725 IP in HeLa cell lysate Lane 3: Mouse IgG instead of NBP3-32725 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL

Immunohistochemistry: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Mouse anti-PCNA antibody (NBP3-32725) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32725) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: PCNA Antibody (A6-G11-R) [NBP3-32725] - Immunocytochemistry analysis of HeLa cells labeling PCNA with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCNA antibody (NBP3-32725) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

Western Blot: PCNA Antibody (A6-G11-R) [NBP3-32725] - Western blot analysis of PCNA on different lysates with Mouse anti-PCNA antibody (NBP3-32725) at 1/20,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HCT 116 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: RAW264.7 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: L6 cell lysate (15 µg/Lane) Lane 8: Mouse spleen tissue lysate (15 µg/Lane) Lane 9: Rat spleen tissue lysate (15 µg/Lane) Predicted band size: 29 kDa Observed band size: 34 kDa Exposure time: 11 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-78712

PCNA Antibody (BLR075G)

100 uL

NBP2-78712

Western Blot: PCNA Antibody (BLR075G) [NBP2-78712] - Detection of human PCNA by WB of A-549, Jurkat, HEK293T, RKO, K-562, MCF-7, HeLa, U2OS, TCMK-1, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-GAPDH (NB300-322).

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Antibody: Rabbit anti-PCNA recombinant monoclonal antibody [BLR075G]Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI.

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Human ovarian carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Human prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.

Immunohistochemistry-Paraffin: PCNA Antibody (BLR075G) [NBP2-78712] - Hhuman OVCAR-3 cells by ICC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-44731

PCNA Antibody (PCNA/694)

0.1 mg

NBP2-44731

Western Blot: PCNA Antibody (PCNA/694) [NBP2-44731] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)

Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) [NBP2-44731] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)

Flow Cytometry: PCNA Antibody (PCNA/694) [NBP2-44731] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Reactivity:

Hu, Ca

Species Glossary

Reactivity: Hu, Ca

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-47835AF350
Alexa Fluor 405	NBP2-47835AF405
Alexa Fluor 488	NBP2-47835AF488
Alexa Fluor 532	NBP2-47835AF532
Alexa Fluor 594	NBP2-47835AF594
Alexa Fluor 647	NBP2-47835AF647
Alexa Fluor 700	NBP2-47835AF700
Alexa Fluor 750	NBP2-47835AF750
Allophycocyanin	NBP2-47835APC
Biotin	NBP2-47835B
CoraFluor 1	NBP2-47835CL1
DyLight 350	NBP2-47835UV
DyLight 405	NBP2-47835V
DyLight 488	NBP2-47835G
DyLight 550	NBP2-47835R
DyLight 594	NBP2-47835DL594
DyLight 650	NBP2-47835C
DyLight 680	NBP2-47835FR
DyLight 755	NBP2-47835IR
FITC	NBP2-47835F
HRP	NBP2-47835H
Janelia Fluor 525	NBP2-47835JF525
Janelia Fluor 549	NBP2-47835JF549
Janelia Fluor 585	NBP2-47835JF585
Janelia Fluor 635	NBP2-47835JF635
Janelia Fluor 646	NBP2-47835JF646
Janelia Fluor 669	NBP2-47835JF669
PE	NBP2-47835PE
PerCP	NBP2-47835PCP
mFluor Violet 450 SE	NBP2-47835MFV450
mFluor Violet 500 SE	NBP2-47835MFV500
mFluor Violet 610 SE	NBP2-47835MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-47835-0.1mg
IHC-Prediluted	NBP2-48284

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