| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9167-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4517-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4517-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61658-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61657-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50529-0.025ml | |||
![N/A Neutrophil Elastase/ELA2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Neutrophil_Elastase_DY451705_ELISA_2461.jpg "N/A Neutrophil Elastase/ELA2 [Biotin]")
![Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Western-Blot-MAB9167-img0001.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Azide-Free-Immunocytochemistry-MAB9167-img0006.jpg)
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils .](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Immunohistochemistry-MAB9167-img0002.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - THP-1 human acute monocytic leukemia cell line treated with 3 uM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB9167, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-950334-Azide-Free-Flow-Cytometry-MAB9167-img0005.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Western blot analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Western-Blot-NBP2-66972-img0009.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Western blot analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - ICC staining of Neutrophil Elastase in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0010.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0011.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of HepG2 cells with Neutrophil Elastase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibod](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Flow-Cytometry-NBP2-66972-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: Mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Western-Blot-NBP2-66972-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0004.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of paraffin-embedded human spleen tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0005.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of paraffin-embedded human tonsil tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0006.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Immunohistochemical analysis of paraffin-embedded mouse bone tissue using anti-Neutrophil Elastase/ELA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0007.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0005.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Immunohistochemistry-NBP2-61658-img0003.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Flow-Cytometry-NBP2-61658-img0002.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0004.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-Western-Blot-NBP2-61658-img0006.jpg)
![ELISA: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6B10-ELISA-NBP2-61658-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of extracts of various cell lines, using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-8X4E6-Western-Blot-NBP3-16724-img0003.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of extracts of various cell lines, using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of human spleen using Neutrophil Elastase/ELA2 Rabbit mAb (NBP3-16724) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-8X4E6-Immunohistochemistry-NBP3-16724-img0002.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Mouse bone marrow using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-8X4E6-Immunohistochemistry-Paraffin-NBP3-16724-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6G6-Western-Blot-NBP2-61657-img0004.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis of K562 cells using ELANE mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6G6-Flow-Cytometry-NBP2-61657-img0002.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6G6-Western-Blot-NBP2-61657-img0003.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mouse mAb against HL-60 (1), THP-1 (2), MOLT4 (3), C6 (4), and K562 (5) cell lysate.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6G6-Western-Blot-NBP2-61657-img0005.jpg)
![ELISA: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-6B6G6-ELISA-NBP2-61657-img0001.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining in human bone marrow and cerebral cortex tissues using anti-ELANE antibody. Corresponding ELANE RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining in human bone marrow and cerebral cortex tissues using anti-ELANE antibody. Corresponding ELANE RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0003.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0002.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-39A-Flow-Cytometry-NB100-64392-img0001.jpg "Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.")