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Limb Bud Formation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Limb Bud Formation Pathway and Congenital Abnormality, Overlying Behavior, Teratogenic Effect, Polydactyly, Arthritis. The study of the Limb Bud Formation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Limb Bud Formation Pathway has been researched in relation to Limb Development, Localization, Cell Death, Myoblast Migration, Cell Migration. The Limb Bud Formation Pathway complements our catalog of research reagents including antibodies and ELISA kits against FGF10, FGF8, SHH, TNF, BMP4.

Top Research Reagents

We have 2395 products for the study of the Limb Bud Formation Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP3-23662
Flow Cytometric Analysis of PFA-fixed HeLa cells. LDB2 antibody (PCRP-LDB2-1B10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Formalin-fixed, paraffin-embedded human tonsil stained with LDB2 antibody (PCRP-LDB2-1B10). Inset: PBS instead of primary antibody; secondary only negative control.

Mouse Monoclonal
Species Human
Applications Flow, ICC/IF, IHC-P

NBP1-86103
Immunohistochemistry-Paraffin: ZP2 Antibody [NBP1-86103] - Staining of human endometrium shows no positivity in glandular cells as expected.Immunohistochemistry-Paraffin: ZP2 Antibody [NBP1-86103] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NBP1-87158
Immunohistochemistry-Paraffin: ALDH1A2 Antibody [NBP1-87158] - Staining in human endometrium and skin tissues . Corresponding ALDH1A2 RNA-seq data are presented for the same tissues.Western Blot: ALDH1A2 Antibody [NBP1-87158] - Analysis in human cell line K562.

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

4 Publications
AF143
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (<a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

27 Publications
AF2085
Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, IHC, ChIP

     2 Reviews

240 Publications
MAB7547
Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Osterix/Sp7 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, ICC

     1 Review

4 Publications
7460-F4/CF


Species Human
Applications BA

2 Publications
464-SH
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).


Species Mouse
Applications BA

65 Publications
DVE00
N/A VEGF [HRP]N/A VEGF [HRP]


Species Human
Applications ELISA

674 Publications
423-F8
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.


Species Human, Mouse
Applications BA

     1 Review

60 Publications
345-FG
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED<SUB>50</SUB> for this effect is 20‑100 ng/mL. 1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.


Species Human
Applications BA

98 Publications
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

803 Publications
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

504 Publications
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Mouse Monoclonal
Species Human, Canine, Monkey
Applications WB, IHC, IHC-P

6 Publications
NBP2-85889
Western Blot: TBX4 Antibody [NBP2-85889] - WB Suggested Anti-TBX4 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysateWestern Blot: TBX4 Antibody [NBP2-85889] - Host: Rabbit. Target: TBX4. Positive control (+): Human lung (LU). Negative control (-): MCF7 (N10). Antibody concentration: 1ug/ml

Rabbit Polyclonal
Species Human
Applications WB

NB600-1287
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

7 Publications
H00002263-M01
Western Blot: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of FGFR2 expression in transfected 293T cell line by FGFR2 monoclonal antibody (M01), clone 1G3.Lane 1: FGFR2 transfected lysate (Predicted MW: 92 KDa).Lane 2: Non-transfected lysate.Immunohistochemistry-Paraffin: FGFR2 Antibody (1G3) [H00002263-M01] - Analysis of monoclonal antibody to FGFR2 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. Antibody concentration 5 ug/ml.

Mouse Monoclonal
Species Human, Bovine
Applications WB, ELISA, ICC/IF

13 Publications
H00002523-P01
12.5% SDS-PAGE Stained with Coomassie Blue.


Species Human
Applications WB, ELISA, PA