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Osteoblast Differentiation Pathway Bioinformatics

Osteoblasts are the cells that are responsible for bone formation. Conversely, osteoclasts are cells that break down bone tissue. Osteoblasts come from immature progenitor cells called osteoprogenitors, which are induced by various growth factors, including bone morphogenetic proteins (BMPs), fibroblast growth factor (FGF), platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-beta). Together, BMPs, FGF, PDGF and TGF-beta increase bone production. In a recent study, it was reported that hESC treatment with BMP4 and FGF2 enhanced osteoblast differentiation (PMID: 23206696). The Wnt/beta-Catenin Pathway may play a role in osteoblast differentiation (PMID: 17572649).

Top Research Reagents

We have 2148 products for the study of the Osteoblast Differentiation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free
NB110-3638
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     1 Review

15 Publications
PDLIM3 Antibody (HL2075) - Azide and BSA Free
NBP3-25358
Immunohistochemistry-Paraffin: PDLIM3 Antibody (HL2075) - Azide and BSA Free [NBP3-25358] - ALP/PDLIM3 antibody [HL2075] detects ALP/PDLIM3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ALP/PDLIM3 stained by ALP/PDLIM3 antibody [HL2075] (NBP3-25358) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Paraffin: PDLIM3 Antibody (HL2075) - Azide and BSA Free [NBP3-25358] - ALP/PDLIM3 antibody [HL2075] detects ALP/PDLIM3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. ALP/PDLIM3 stained by ALP/PDLIM3 antibody [HL2075] (NBP3-25358) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

ASRGL1 Antibody
NBP1-89133
Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
NAT10 Antibody
NBP2-32263
Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IP

Osteopontin/OPN Antibody [Unconjugated]
AF808
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

Goat Polyclonal
Species Mouse
Applications WB, IHC, ELISA(Cap)

     2 Reviews

206 Publications
Osterix/Sp7 Antibody (764704)
MAB7547
Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Osterix/Sp7 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, ICC

     1 Review

5 Publications
RUNX2/CBFA1 Antibody (232902) [Unconjugated]
MAB2006
RUNX2/CBFA1 was detected in immersion fixed U2OS human osteosarcoma cell line using Rat Anti-Human RUNX2/CBFA1 Monoclonal Antibody (Catalog # MAB2006) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Rat Monoclonal
Species Human
Applications ICC

     1 Review

28 Publications
Osteocalcin Antibody (190125)
MAB1419
Osteocalcin was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=Osteocalcin was detected in human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Mouse Monoclonal
Species Human, Rat
Applications IHC, CyTOF-ready, ICC

     3 Reviews

56 Publications
IBSP/Sialoprotein II [Unconjugated]
4014-SP
Recombinant Human IBSP/Sialoprotein II Protein (Catalog # 4014-SP) supports the adhesion of MCF‑7 human breast cancer cells. The ED<sub>50</sub> for this effect is 0.0600-0.600 µg/mL.


Species Human
Applications BA

5 Publications
Osteoprotegerin/TNFRSF11B [Biotin]
DY805
N/A Osteoprotegerin/TNFRSF11B [Biotin]


Species Human
Applications ELISA

89 Publications
SLPI [HRP]
DPI00
N/A SLPI [HRP]N/A SLPI [HRP]


Species Human
Applications ELISA

37 Publications
CCL27/CTACK [HRP]
DCC270
N/A CCL27/CTACK [HRP]N/A CCL27/CTACK [HRP]


Species Human
Applications ELISA

10 Publications
BMP-2 [Unconjugated]
355-BM
Recombinant human/mouse/rat BMP-2 (<a class=NoLineLink href=


Species Human, Mouse, Rat
Applications BA

190 Publications
TRANCE/TNFSF11/RANK L [Unconjugated]
462-TEC
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED<SUB>50</SUB> for this effect is 0.5-2 ng/mL. 1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.


Species Mouse
Applications BA

90 Publications
BMP-4 [Unconjugated]
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

519 Publications
PTH Antibody (918462) [Unconjugated]
MAB7665
Western blot shows lysates of human parathyroid tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=PTH was detected in immersion fixed paraffin-embedded sections of human parathyroid gland using Mouse Anti-Human PTH Monoclonal Antibody (Catalog # MAB7665) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, IHC