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Leukocyte Migration Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Leukocyte Migration Pathway and Inflammation, Tissue Adhesions, Neoplasms, Delayed Hypersensitivity, Malignant Neoplasms. The study of the Leukocyte Migration Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Leukocyte Migration Pathway has been researched in relation to Immune Response, Chemotaxis, Hypersensitivity, Cell Migration, Cell Adhesion. The Leukocyte Migration Pathway complements our catalog of research reagents including antibodies and ELISA kits against TNF, ITGB2, ICAM1, CCL2, LIF.

Top Research Reagents

We have 6805 products for the study of the Leukocyte Migration Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

IL-6 [HRP]
D6050B
N/A IL-6 [HRP]N/A IL-6 [HRP]


Species Human

766 Publications
VSX1 Antibody
NBP3-25228
Staining of human cell line U2OS shows localization to nucleoli.

Rabbit Polyclonal
Species Human
Applications ICC/IF

HPD Antibody
NBP1-89366
Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Analysis in human liver and endometrium tissues. Corresponding HPD RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human endometrium, kidney, liver and squamous epithelia using Anti-HPD antibody NBP1-89366 (A) shows similar protein distribution across tissues to independent antibody NBP2-32657 (B).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

Myeloperoxidase/MPO Antibody [Unconjugated]
AF3667
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

     3 Reviews

161 Publications
L-Selectin/CD62L Antibody (4G8) [Unconjugated]
BBA24
PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # <a class=NoLineLink href='https://www.rndsystems.com/product_results.aspx?k=FAB100A'>FAB100A</a>) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) or (B) isotype control antibody (Catalog # <a class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications Flow, CyTOF-ready, ELISA(Cap)

9 Publications
ICAM-1/CD54 Antibody [Unconjugated]
AF796
ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary delta / delta ep2 keratinocytes stimulated with EGF and/or TNF alpha and IL1 beta for 15 min. (B) Increased cytokine and chemokine production in primary delta / delta ep2 keratinocytes treated with EGF, TNF alpha and IL1 beta for 24 hr. Cytokine and chemokine production was determined by multiplex analysis, except for TSLP which was quantified by ELISA. Data represent mean ± SEM of 3–5 biological replicates. (C–D) Cells were pretreated with D-JNKI1 inhibitors prior to stimulation with EGF, TNF alpha and IL1 beta for 15 min (C) or 24 hr (D). Data represent the mean ± SEM of technical replicates (n = 3). (E–F) Effect of shRNA-mediated Mlk3 silencing on ERK and JNK phosphorylation and ICAM1 expression (E; stimulation with EGF, TNF alpha and IL1 beta for 15 min) and on the expression of Ccl2 and Tslp mRNA (F; stimulation with EGF, TNF alpha and IL1 beta for 24 hr) by F/F2 and delta / delta ep2 keratinocytes. shRen, shRNA targeting Renilla, used as a control; sh1 and sh2, targeting Mlk3, binding sites nucleotide 2266–2285 and 2383–2402, respectively. The shRNAs were encoded by lentiviral vectors coexpressing GFP. GFP immunoblots are shown to confirm similar levels of infection in all samples. Data represent mean ± SEM of 4 biological replicates. Each keratinocyte culture represents a pool of three mice. Immunoblots are representative of three independent experiments. p1 = 0.041, p2 = 0.040, p3 = 1.89E-4, p4 = 0.018, p5 = 0.046, p6 = 0.020, p7 = 0.008, p8 = 0.016, p9 = 0.001, p10 = 0.018, p11 = 3.23E-4, p12 = 1.47E-4, p13 = 0.007, p14 = 0.03, p15 = 0.035, p16 = 0.023 and p17 = 0.046.DOI:https://dx.doi.org/10.7554/eLife.14012.018Compound knockdown (KD2) of BRAF and RAF1 induce the expression of inflammation markers by HaCat cells in a MLK3/JNK-dependent manner.(A) Reduced ERK and increased JNK/p38 activation in BRAF and RAF1 knockdown (KD2) HaCat cells stimulated with EGF, TNF alpha and IL1 beta for 15 min. (B) D-JNKI1 reduces ICAM1 and CCL2 (n = 4) expression in KD2 cells treated with TNF alpha . (C) MEKi induces ICAM1 and CCL2 (n = 3) expression in RAF1KD cells treated with TNF alpha . In (B–C), ICAM1 expression was measured after a 3 hr, CCL2 expression after a 24 hr treatment with TNF alpha . (D) Effect of MLK3 silencing on ERK and JNK phosphorylation in WT and KD2 cells stimulated as in (A). MLK3 was silenced using a pool of oligonucleotides targeting the following regions: 686–704; 1489–1507; 2122–2138; and 2348–2366. MLK3 KD cells stimulated as in (B–C) show a decrease in JNK activation, ICAM1 and CCL2 (n = 7) expression. Immunoblots are representative of three independent experiments. qPCR data represent mean ± SEM of three independent experiments run in duplicates (p1 = 4.62E-4, p2 = 0.013, p3 = 0.050, p4 = 8.60E-8, p5 = 0.050, p6 = 0.001, p7 = 0.001 and p8 = 0.012).DOI:https://dx.doi.org/10.7554/eLife.14012.019 Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/14012), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Mouse
Applications WB, IHC, AdBlk

     6 Reviews

85 Publications
Integrin alpha L/CD11a Antibody (345913) [Unconjugated]
MAB3595
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of PBMC, Placenta, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 220 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB3595). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Mouse Monoclonal
Species Human
Applications WB, Simple Western, Flow

     1 Review

3 Publications
Integrin beta 2/CD18 Antibody [Unconjugated]
AF1730
Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=Integrin  beta 2/CD18 was detected in immersion fixed THP‑1 human acute monocytic leukemia cells (Positive) & absent in RT‑4 human urinary bladder transitional cell papilloma (Negative) using Goat Anti-Human Integrin  beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=NoLineLink href=

Goat Polyclonal
Species Human
Applications Flow, AdBlk, CyTOF-ready

14 Publications
Lymphotoxin beta R/TNFRSF3 Antibody
AF629

Goat Polyclonal
Species Human
Applications WB, Flow, CyTOF-ready

10 Publications
LIF [Unconjugated]
7734-LF


Species Human
Applications BA

30 Publications
CTLA-4 [Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED<sub>50</sub> for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # <a class=


Species Human
Applications BA

3 Publications
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]N/A CCL2/JE/MCP-1 [HRP]


Species Human
Applications ELISA

251 Publications
IFN-gamma [Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285-IF) has a molecular weight (MW) of 34.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.


Species Human
Applications BA

     2 Reviews

446 Publications
TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

807 Publications
CXCL8/IL-8 [HRP]
D8000C
N/A CXCL8/IL-8 [HRP]N/A CXCL8/IL-8 [HRP]


Species Human
Applications ELISA

437 Publications
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]N/A CCL5/RANTES [HRP]


Species Human
Applications ELISA

100 Publications
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]


Species Mouse
Applications ELISA

291 Publications
Ago2/eIF2C2 Antibody (JF0992)
NBP2-67121
Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysateLane 2: Hela cell lysateImmunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
Lamin B Receptor Antibody
H00003930-B01P
Western Blot: Lamin B Receptor Antibody [H00003930-B01P] - Analysis of LBR expression in transfected 293T cell line by LBR polyclonal antibody. Lane 1: LBR transfected lysate(67.65 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody [H00003930-B01P] - Analysis of purified antibody to LBR on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

     1 Review